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Attenuating Immune Response of Macrophage by Enhancing Hydrophilicity of Ti Surface
Joint Authors
Zhang, Xuehui
Meng, Song
Dai, Xiaohan
Wei, Yan
Mo, Xiaoju
Liu, Xing
Zhang, Li
Deng, Xuming
Liang, Xu-Deng
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Immune responses can determine the in vivo fate of implanted materials.
The strategy for developing implants has shifted towards using materials with immunomodulatory activity.
However, the immunoregulatory effect of hydrophilicity of titanium surface on the macrophage behavior and its underlying mechanism remain poorly understood.
Here, the Ti surface hydrophilicity-dependent behavior of murine RAW264.7 macrophages was investigated in vitro.
Two laboratory models with significantly different surface hydrophilicity and similar roughness were established with Ti-polished and Ti-H2O2 surfaces.
The results of cell morphology observation showed that the Ti-H2O2 surface yielded enhanced cell adhesion and less multinucleated cell formation.
CCK-8 assay indicated that the growth rate of macrophage on Ti-H2O2 surface is higher than that of Ti-polished.
ELISA assay result revealed lower level of proinflammatory factor TNF-α and higher level of anti-inflammatory factor IL-10 on the Ti-H2O2 surface compared to Ti-polished.
Subsequently, immunofluorescence and western blotting analysis showed that activation of the NF-κB-TNF-α pathway might be involved in the modulation of the immune response by surface hydrophilicity.
Together, these results suggested that relative high hydrophilic Ti surface might attenuate the immune response of macrophage by activating NF-κB signaling.
These findings could provide new insights into designing implant devices for orthopedic applications.
American Psychological Association (APA)
Dai, Xiaohan& Wei, Yan& Zhang, Xuehui& Meng, Song& Mo, Xiaoju& Liu, Xing…[et al.]. 2015. Attenuating Immune Response of Macrophage by Enhancing Hydrophilicity of Ti Surface. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069256
Modern Language Association (MLA)
Dai, Xiaohan…[et al.]. Attenuating Immune Response of Macrophage by Enhancing Hydrophilicity of Ti Surface. Journal of Nanomaterials No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1069256
American Medical Association (AMA)
Dai, Xiaohan& Wei, Yan& Zhang, Xuehui& Meng, Song& Mo, Xiaoju& Liu, Xing…[et al.]. Attenuating Immune Response of Macrophage by Enhancing Hydrophilicity of Ti Surface. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069256
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069256