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Radiosensitizing and Hyperthermic Properties of Hyaluronan Conjugated, Dextran-Coated Ferric Oxide Nanoparticles: Implications for Cancer Stem Cell Therapy
Joint Authors
Thapa, Ranjeeta
Galoforo, Sandra
El-dakdouki, Mohammad H.
Wilson, Thomas G.
Huang, Xuefei
Wilson, George D.
Roth, Bradley J.
Kandel, Sunil M.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Cytotoxicity, radiosensitivity, and hyperthermia sensitivity of hyaluronan-mediated dextran-coated super paramagnetic iron oxide nanoparticles (HA-DESPIONs) were assessed in CD44-expressing head and neck squamous cell carcinoma (HNSCC) cell lines at clinically relevant radiation dose and temperatures.
Low-passage HNSCC cells were exposed to HA-DESPIONs and cytotoxicity was assessed using MTT assay.
Radiosensitizing properties of graded doses of HA-DESPIONs were assessed in both unsorted and CD44-sorted cells using clonogenic assay in combination with 2 Gy exposure to X-rays.
Hyperthermia-induced toxicity was measured at 40°C, 41°C, and 42°C using clonogenic assay.
Cell death was assessed 24 hours after treatment using a flow cytometry-based apoptosis analysis.
Results showed that HA-DESPIONs were nontoxic at moderate concentrations and did not directly radiosensitize the cell lines.
Further, there was no significant difference in the radiosensitivity of C D 44 h i g h and C D 44 l o w cells.
However, HA-DESPIONs enhanced the effect of hyperthermia which resulted in reduced cell survival that appeared to be mediated through apoptosis.
We demonstrated that HA-DESPIONs are nontoxic and although they do not enhance radiation sensitivity, they did increase the effect of local hyperthermia.
These results support further development of drug-attached HA-DESPIONs in combination with radiation for targeting cancer stem cells (CSCs) and the development of an alternating magnetic field approach to activate the HA-DESPIONs attached to CSCs.
American Psychological Association (APA)
Thapa, Ranjeeta& Galoforo, Sandra& Kandel, Sunil M.& El-dakdouki, Mohammad H.& Wilson, Thomas G.& Huang, Xuefei…[et al.]. 2015. Radiosensitizing and Hyperthermic Properties of Hyaluronan Conjugated, Dextran-Coated Ferric Oxide Nanoparticles: Implications for Cancer Stem Cell Therapy. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1069364
Modern Language Association (MLA)
Thapa, Ranjeeta…[et al.]. Radiosensitizing and Hyperthermic Properties of Hyaluronan Conjugated, Dextran-Coated Ferric Oxide Nanoparticles: Implications for Cancer Stem Cell Therapy. Journal of Nanomaterials No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1069364
American Medical Association (AMA)
Thapa, Ranjeeta& Galoforo, Sandra& Kandel, Sunil M.& El-dakdouki, Mohammad H.& Wilson, Thomas G.& Huang, Xuefei…[et al.]. Radiosensitizing and Hyperthermic Properties of Hyaluronan Conjugated, Dextran-Coated Ferric Oxide Nanoparticles: Implications for Cancer Stem Cell Therapy. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1069364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069364