Silica Sol-Gel Entrapment of the Enzyme Chloroperoxidase
Joint Authors
Le, Tuan
Chan, Selina
Ebaid, Bassem
Sommerhalter, Monika
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The enzyme chloroperoxidase (CPO) was immobilized in silica sol-gel beads prepared from tetramethoxysilane.
The average pore diameter of the silica host structure (~3 nm) was smaller than the globular CPO diameter (~6 nm) and the enzyme remained entrapped after sol-gel maturation.
The catalytic performance of the entrapped enzyme was assessed via the pyrogallol peroxidation reaction.
Sol-gel beads loaded with 4 μg CPO per mL sol solution reached 9–12% relative activity compared to free CPO in solution.
Enzyme kinetic analysis revealed a decrease in kcat but no changes in KM or KI.
Product release or enzyme damage might thus limit catalytic performance.
Yet circular dichroism and visible absorption spectra of transparent CPO sol-gel sheets did not indicate enzyme damage.
Activity decline due to methanol exposure was shown to be reversible in solution.
To improve catalytic performance the sol-gel protocol was modified.
The incorporation of 5, 20, or 40% methyltrimethoxysilane resulted in more brittle sol-gel beads but the catalytic performance increased to 14% relative to free CPO in solution.
The use of more acidic casting buffers (pH 4.5 or 5.5 instead of 6.5) resulted in a more porous silica host reaching up to 18% relative activity.
American Psychological Association (APA)
Le, Tuan& Chan, Selina& Ebaid, Bassem& Sommerhalter, Monika. 2015. Silica Sol-Gel Entrapment of the Enzyme Chloroperoxidase. Journal of Nanotechnology،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1069546
Modern Language Association (MLA)
Le, Tuan…[et al.]. Silica Sol-Gel Entrapment of the Enzyme Chloroperoxidase. Journal of Nanotechnology No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1069546
American Medical Association (AMA)
Le, Tuan& Chan, Selina& Ebaid, Bassem& Sommerhalter, Monika. Silica Sol-Gel Entrapment of the Enzyme Chloroperoxidase. Journal of Nanotechnology. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1069546
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069546