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Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis
Joint Authors
Chen, Li
Tao, Yong
Feng, Jing
Jiang, Yan Rong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-27
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Pericytes are a population of cells that participate in normal vessel architecture and regulate permeability.
Apelin, as the endogenous ligand of G protein-coupled receptor APJ, participates in a number of physiological and pathological processes.
To date, the effect of apelin on pericyte is not clear.
Our study aimed to investigate the potential protection mechanisms of apelin, with regard to primary rat retinal pericytes under hypoxia.
Immunofluorescence staining revealed that pericytes colocalized with APJ in the fibrovascular membranes dissected from proliferative diabetic retinopathy patients.
In the in vitro studies, we first demonstrated that the expression of apelin/APJ was upregulated in pericytes under hypoxia, and apelin increased pericytes proliferation and migration.
Moreover, knockdown of apelin in pericyte was achieved via lentivirus-mediated RNA interference.
After the inhibition of apelin, pericytes proliferation was inhibited significantly in hypoxia culture condition.
Furthermore, exogenous recombinant apelin effectively prevented hypoxia-induced apoptosis through downregulating active-caspase 3 expression and increasing the ratio of B cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax) in pericytes.
These results suggest that apelin suppressed hypoxia-induced pericytes injury, which indicated that apelin could be a potential therapeutic target for retinal angiogenic diseases.
American Psychological Association (APA)
Chen, Li& Tao, Yong& Feng, Jing& Jiang, Yan Rong. 2015. Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis. Journal of Ophthalmology،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1069666
Modern Language Association (MLA)
Chen, Li…[et al.]. Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis. Journal of Ophthalmology No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1069666
American Medical Association (AMA)
Chen, Li& Tao, Yong& Feng, Jing& Jiang, Yan Rong. Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis. Journal of Ophthalmology. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1069666
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069666