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Histological Characterization of the Dicer1 Mutant Zebrafish Retina
Joint Authors
Shu, Xinhua
Akhtar, Saeed
Patnaik, Sarita Rani
Kotapati Raghupathy, Rakesh
Al-Mubrad, Turki M.
Craft, John A.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
DICER1, a multidomain RNase III endoribonuclease, plays a critical role in microRNA (miRNA) and RNA-interference (RNAi) functional pathways.
Loss of Dicer1 affects different developmental processes.
Dicer1 is essential for retinal development and maintenance.
DICER1 was recently shown to have another function of silencing the toxicity of Alu RNAs in retinal pigment epithelium (RPE) cells, which are involved in the pathogenesis of age related macular degeneration.
In this study, we characterized a Dicer1 mutant fish line, which carries a nonsense mutation (W1457Ter) induced by N-ethyl-N-nitrosourea mutagenesis.
Zebrafish DICER1 protein is highly conserved in the evolution.
Zebrafish Dicer1 is expressed at the earliest stages of zebrafish development and persists into late developmental stages; it is widely expressed in adult tissues.
Homozygous Dicer1 mutant fish (DICER 1 W1457Ter/W1457Ter ) have an arrest in early growth with significantly smaller eyes and are dead at 14–18 dpf.
Heterozygous Dicer1 mutant fish have similar retinal structure to that of control fish; the retinal pigment epithelium (RPE) cells are normal with no sign of degeneration at the age of 20 months.
American Psychological Association (APA)
Akhtar, Saeed& Patnaik, Sarita Rani& Kotapati Raghupathy, Rakesh& Al-Mubrad, Turki M.& Craft, John A.& Shu, Xinhua. 2015. Histological Characterization of the Dicer1 Mutant Zebrafish Retina. Journal of Ophthalmology،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069695
Modern Language Association (MLA)
Akhtar, Saeed…[et al.]. Histological Characterization of the Dicer1 Mutant Zebrafish Retina. Journal of Ophthalmology No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1069695
American Medical Association (AMA)
Akhtar, Saeed& Patnaik, Sarita Rani& Kotapati Raghupathy, Rakesh& Al-Mubrad, Turki M.& Craft, John A.& Shu, Xinhua. Histological Characterization of the Dicer1 Mutant Zebrafish Retina. Journal of Ophthalmology. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069695
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069695