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Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation
Joint Authors
Haritoglou, C.
Mayer, Wolfgang J.
Schumann, Ricarda
Compera, Denise
Kampik, Anselm
Entchev, Enrico
Hagenau, Felix
Ziada, Jean
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Purpose.
To describe morphology of lamellar hole-associated epiretinal proliferation (LHEP) removed from eyes with lamellar macular holes (LMH).
Methods.
Based on optical coherence tomography data, 10 specimens of LHEP were removed from 10 eyes with LMH during standard vitrectomy.
Specimens were prepared for correlative light and electron microscopy (CLEM) using an immunonanogold particle of 1.4 nm diameter that was combined with a fluorescein moiety, both having been attached to a single antibody fragment.
As primary antibodies, we used antiglial fibrillary acidic protein (GFAP), anti-CD45, anti-CD64, anti-α-smooth muscle actin (α-SMA), and anticollagen type I and type II.
Results.
In LHEP, GFAP-positive cells possess ultrastructural characteristics of fibroblasts and hyalocytes.
They represent the major cell types and were densely packed in cell agglomerations on vitreous collagen strands.
Epiretinal cells of LHEP rarely demonstrated contractive properties as α-SMA-positive myofibroblasts were an infrequent finding.
Conclusion.
CLEM indicates that epiretinal cells in LHEP might originate from the vitreous and that remodelling processes of vitreous collagen may play an important role in pathogenesis of eyes with LMH.
American Psychological Association (APA)
Compera, Denise& Entchev, Enrico& Haritoglou, C.& Mayer, Wolfgang J.& Hagenau, Felix& Ziada, Jean…[et al.]. 2015. Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation. Journal of Ophthalmology،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069743
Modern Language Association (MLA)
Compera, Denise…[et al.]. Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation. Journal of Ophthalmology No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1069743
American Medical Association (AMA)
Compera, Denise& Entchev, Enrico& Haritoglou, C.& Mayer, Wolfgang J.& Hagenau, Felix& Ziada, Jean…[et al.]. Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation. Journal of Ophthalmology. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069743
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069743