Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits
Joint Authors
Prause, J. U.
La Cour, M.
Mandal, Nakul
Lewis, Geoffrey P.
Fisher, Steven K.
Heegaard, Steffen
Vorum, Henrik
Honoré, Bent
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose.
The pathogenesis of rhegmatogenous retinal detachment (RRD) remains incompletely understood, with no clinically effective treatment for potentially severe complications such as photoreceptor cell death and proliferative vitreoretinopathy.
Here we investigate the protein profile of the vitreous following experimental retinal detachment using a comparative proteomic based approach.
Materials and Methods.
Retinal detachment was created in the right eyes of six New Zealand red pigmented rabbits.
Sham surgery was undertaken in five other rabbits that were used as controls.
After seven days the eyes were enucleated and the vitreous was removed.
The vitreous samples were evaluated with two-dimensional polyacrylamide gel electrophoresis and the differentially expressed proteins were identified with tandem mass spectrometry.
Results.
Ten protein spots were found to be at least twofold differentially expressed when comparing the vitreous samples of the sham and retinal detachment surgery groups.
Protein spots that were upregulated in the vitreous following retinal detachment were identified as albumin fragments, and those downregulated were found to be peroxiredoxin 2, collagen-Iα1 fragment, and α-1-antiproteinase F.
Conclusions.
Proteomic investigation of the rabbit vitreous has identified a set of proteins that help further our understanding of the pathogenesis of rhegmatogenous retinal detachment and its complications.
American Psychological Association (APA)
Mandal, Nakul& Lewis, Geoffrey P.& Fisher, Steven K.& Heegaard, Steffen& Prause, J. U.& La Cour, M.…[et al.]. 2015. Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits. Journal of Ophthalmology،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1069779
Modern Language Association (MLA)
Mandal, Nakul…[et al.]. Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits. Journal of Ophthalmology No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1069779
American Medical Association (AMA)
Mandal, Nakul& Lewis, Geoffrey P.& Fisher, Steven K.& Heegaard, Steffen& Prause, J. U.& La Cour, M.…[et al.]. Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits. Journal of Ophthalmology. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1069779
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069779