Blockade of Vascular Endothelial Growth Factor Receptor 1 Prevents Inflammation and Vascular Leakage in Diabetic Retinopathy
Joint Authors
He, Jianbo
Wang, Hong
Liu, Ying
Li, Wen
Kim, Dorothy
Huang, Hu
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Diabetic retinopathy (DR) is a leading cause of blindness in working age adults.
The objective of this study is to investigate the effects of vascular endothelial growth factor receptor 1 (VEGFR1) blockade on the complications of DR.
Experimental models of diabetes were induced with streptozotocin (STZ) treatment or Insulin2 gene mutation (Akita) in mice.
Protein expression and localization were examined by western blots (WB) and immunofluorescence (IF).
mRNA expression was quantified by PCR array and real-time PCR.
The activity of VEGFR1 signaling was blocked by a neutralizing antibody called MF1.
Vascular leakage was evaluated by measuring the leakage of [3H]-mannitol tracer into the retina and the IF staining of albumin.
VEGFR1 blockade significantly inhibited diabetes-related vascular leakage, leukocytes-endothelial cell (EC) adhesion (or retinal leukostasis), expression of intercellular adhesion molecule- (ICAM-) 1 protein, abnormal localization and degeneration of the tight junction protein zonula occludens- (ZO-) 1, and the cell adhesion protein vascular endothelial (VE) cadherin.
In addition, VEGFR1 blockade interfered with the gene expression of 10 new cytokines and chemokines: cxcl10, il10, ccl8, il1f6, cxcl15, ccl4, il13, ccl6, casp1, and ccr5.
These results suggest that VEGFR1 mediates complications of DR and targeting this signaling pathway represents a potential therapeutic strategy for the prevention and treatment of DR.
American Psychological Association (APA)
He, Jianbo& Wang, Hong& Liu, Ying& Li, Wen& Kim, Dorothy& Huang, Hu. 2015. Blockade of Vascular Endothelial Growth Factor Receptor 1 Prevents Inflammation and Vascular Leakage in Diabetic Retinopathy. Journal of Ophthalmology،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1069786
Modern Language Association (MLA)
He, Jianbo…[et al.]. Blockade of Vascular Endothelial Growth Factor Receptor 1 Prevents Inflammation and Vascular Leakage in Diabetic Retinopathy. Journal of Ophthalmology No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1069786
American Medical Association (AMA)
He, Jianbo& Wang, Hong& Liu, Ying& Li, Wen& Kim, Dorothy& Huang, Hu. Blockade of Vascular Endothelial Growth Factor Receptor 1 Prevents Inflammation and Vascular Leakage in Diabetic Retinopathy. Journal of Ophthalmology. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1069786
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069786