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Sublethal Photothermal Stimulation with a Micropulse Laser Induces Heat Shock Protein Expression in ARPE-19 Cells
Joint Authors
Inagaki, Keiji
Ohkoshi, Kishiko
Ebihara, Nobuyuki
Shuo, Takuya
Katakura, Kanae
Murakami, Akira
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose/Aim of the Study.
Subthreshold micropulse diode laser photocoagulation is an effective treatment for macular edema.
The molecular mechanisms underlying treatment success are poorly understood.
Therefore, we investigated the effects of sublethal laser energy doses on a single layer of densely cultured ARPE-19 cells as a model of the human retinal pigment epithelium (RPE).
Materials and Methods.
A single layer of densely cultured human ARPE-19 cells was perpendicularly irradiated with a micropulse diode laser.
Nonirradiated cells served as controls.
Sublethal laser energy was applied to form a photocoagulation-like area in the cultured cell layers.
Hsp70 expression was evaluated using quantitative polymerase chain reaction and immunocytochemistry.
Results.
Photocoagulation-like areas were successfully created in cultured ARPE-19 cell layers using sublethal laser energy with our laser irradiation system.
Hsp70 mRNA expression in cell layers was induced within 30 min of laser irradiation, peaking at 3 h after irradiation.
This increase was dependent on the number of laser pulses.
Hsp70 upregulation was not observed in untreated cell layers.
Immunostaining indicated that Hsp70 expression occurred concentrically around laser irradiation sites and persisted for 24 h following irradiation.
Conclusion.
Sublethal photothermal stimulation with a micropulse laser may facilitate Hsp70 expression in the RPE without inducing cellular damage.
American Psychological Association (APA)
Inagaki, Keiji& Shuo, Takuya& Katakura, Kanae& Ebihara, Nobuyuki& Murakami, Akira& Ohkoshi, Kishiko. 2015. Sublethal Photothermal Stimulation with a Micropulse Laser Induces Heat Shock Protein Expression in ARPE-19 Cells. Journal of Ophthalmology،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1069821
Modern Language Association (MLA)
Inagaki, Keiji…[et al.]. Sublethal Photothermal Stimulation with a Micropulse Laser Induces Heat Shock Protein Expression in ARPE-19 Cells. Journal of Ophthalmology No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1069821
American Medical Association (AMA)
Inagaki, Keiji& Shuo, Takuya& Katakura, Kanae& Ebihara, Nobuyuki& Murakami, Akira& Ohkoshi, Kishiko. Sublethal Photothermal Stimulation with a Micropulse Laser Induces Heat Shock Protein Expression in ARPE-19 Cells. Journal of Ophthalmology. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1069821
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069821