iTRAQ-Based Proteomic Analysis of Visual Cycle-Associated Proteins in RPE of rd12 Mice before and after RPE65 Gene Delivery
Joint Authors
Zheng, Qinxiang
Ren, Yueping
Tzekov, Radouil
Hua, Shanshan
Li, Minghan
Pang, Jijing
Qu, Jia
Li, Wensheng
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Purpose.
To investigate the iTRAQ-based proteomic changes of visual cycle-associated proteins in RPE of rd12 mice before and after RPE65 gene delivery.
Mehtods.
The right eyes of rd12 mice underwent RPE65 gene delivery by subretinal injection at P14, leaving the left eyes as control.
C57BL/6J mice were served as a wide-type control group.
ERGs were recorded at P42, and RPE-choroid-sclera complex was collected to evaluate the proteomic changes in visual cycle-associated proteins by iTRAQ-based analysis.
Western blot was used to confirm the changes in the differentially expressed proteins of interest.
Results.
ERG parameters improved dramatically at P42 after RPE65 delivery.
The proteomics analysis identified a total 536 proteins with a global false discovery rate of 0.21%, out of which 7 were visual cycle-associated proteins.
RALBP-1, RBP-1, and IRBP were reduced in the untreated rd12 eyes and the former two were improved after gene therapy, confirmed by Western blot analysis.
Conclusions.
RPE65 gene delivery restored retinal function at P42 and modified the expression of other functional proteins implicated in the visual cycle.
The level of RALBP-1 was still below the normal level after gene therapy in rd12 mice, which may explain the delayed dark adaption in LCA patients undergoing similar therapy.
American Psychological Association (APA)
Zheng, Qinxiang& Ren, Yueping& Tzekov, Radouil& Hua, Shanshan& Li, Minghan& Pang, Jijing…[et al.]. 2015. iTRAQ-Based Proteomic Analysis of Visual Cycle-Associated Proteins in RPE of rd12 Mice before and after RPE65 Gene Delivery. Journal of Ophthalmology،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069886
Modern Language Association (MLA)
Zheng, Qinxiang…[et al.]. iTRAQ-Based Proteomic Analysis of Visual Cycle-Associated Proteins in RPE of rd12 Mice before and after RPE65 Gene Delivery. Journal of Ophthalmology No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1069886
American Medical Association (AMA)
Zheng, Qinxiang& Ren, Yueping& Tzekov, Radouil& Hua, Shanshan& Li, Minghan& Pang, Jijing…[et al.]. iTRAQ-Based Proteomic Analysis of Visual Cycle-Associated Proteins in RPE of rd12 Mice before and after RPE65 Gene Delivery. Journal of Ophthalmology. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069886
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069886