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Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran
Joint Authors
Shams, Ehsaneh
Moniri, Rezvan
Firoozeh, Farzaneh
Zibaei, Mohammad
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The purpose of this study was to determine the prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, aac(6′)-Ib-cr, and qepA) among ESBL-producing Klebsiella pneumoniae isolates in Kashan, Iran.
A total of 185 K.
pneumoniae isolates were tested for quinolone resistance and ESBL-producing using the disk diffusion method and double disk synergy (DDST) confirmatory test.
ESBL-producing strains were further evaluated for the blaCTX-M genes.
The PCR method was used to show presence of plasmid-mediated quinolone resistance genes and the purified PCR products were sequenced.
Eighty-seven ESBL-producing strains were identified by DDST confirmatory test and majority (70, 80.5%) of which carried blaCTX-M genes including CTX-M-1 (60%), CTX-M-2 (42.9%), and CTX-M-9 (34.3%).
Seventy-seven ESBL-producing K.
pneumoniae isolates harbored PMQR genes, which mostly consisted of aac(6′)-Ib-cr (70.1%) and qnrB (46.0%), followed by qnrS (5.7%).
Among the 77 PMQR-positive isolates, 27 (35.1%) and 1 (1.3%) carried 2 and 3 different PMQR genes, respectively.
However, qnrA and qepA were not found in any isolate.
Our results highlight high ESBL occurrence with CTX-M type and high frequency of plasmid-mediated quinolone resistance genes among ESBL-producing K.
pneumoniae isolates in Kashan.
American Psychological Association (APA)
Shams, Ehsaneh& Firoozeh, Farzaneh& Moniri, Rezvan& Zibaei, Mohammad. 2015. Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran. Journal of Pathogens،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1069943
Modern Language Association (MLA)
Shams, Ehsaneh…[et al.]. Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran. Journal of Pathogens No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1069943
American Medical Association (AMA)
Shams, Ehsaneh& Firoozeh, Farzaneh& Moniri, Rezvan& Zibaei, Mohammad. Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran. Journal of Pathogens. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1069943
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069943