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Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
Joint Authors
Dmitryjuk, Małgorzata
Łopieńska-Biernat, Elżbieta
Zaobidna, Ewa Anna
Source
Journal of Parasitology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Trehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including Anisakis simplex.
Nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis.
The aim of this study was to investigate the expression of genes encoding the enzymes involved in the metabolism of trehalose and glycogen—trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP), glycogen synthase (GS), and glycogen phosphorylase (GP)—in stage L3 and stage L4 larvae of A.
simplex.
The expression of mRNA all four genes, tps, tpp, gs, and gp, was examined by real-time polymerase chain reaction.
The A.
simplex ribosomal gene (18S) was used as a reference gene.
Enzymatic activity was determined.
The expression of trehalose enzyme genes was higher in L3 than in L4 larvae, but an inverse relationship was noted for the expression of gs and gp genes.
American Psychological Association (APA)
Łopieńska-Biernat, Elżbieta& Zaobidna, Ewa Anna& Dmitryjuk, Małgorzata. 2015. Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex. Journal of Parasitology Research،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069964
Modern Language Association (MLA)
Łopieńska-Biernat, Elżbieta…[et al.]. Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex. Journal of Parasitology Research No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1069964
American Medical Association (AMA)
Łopieńska-Biernat, Elżbieta& Zaobidna, Ewa Anna& Dmitryjuk, Małgorzata. Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex. Journal of Parasitology Research. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069964
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069964