Toxicity Biosensor for Sodium Dodecyl Sulfate Using Immobilized Green Fluorescent Protein Expressing Escherichia coli
Joint Authors
Heng, Lee Yook
Ooi, Lia
Ahmad, Asmat
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Green fluorescent protein (GFP) is suitable as a toxicity sensor due to its ability to work alone without cofactors or substrates.
Its reaction with toxicants can be determined with fluorometric approaches.
GFP mutant gene (C48S/S147C/Q204C/S65T/Q80R) is used because it has higher sensitivity compared to others GFP variants.
A novel sodium dodecyl sulfate (SDS) toxicity detection biosensor was built by immobilizing GFP expressing Escherichia coli in k-Carrageenan matrix.
Cytotoxicity effect took place in the toxicity biosensor which leads to the decrease in the fluorescence intensity.
The fabricated E.
coli GFP toxicity biosensor has a wide dynamic range of 4–100 ppm, with LOD of 1.7 ppm.
Besides, it possesses short response time (<1 min), high reproducibility (0.76% RSD) and repeatability (0.72% RSD, R2>0.98), and long-term stability (46 days).
E.
coli GFP toxicity biosensor has been applied to detect toxicity induced by SDS in tap water, river water, and drinking water.
High recovery levels of SDS indicated the applicability of E.
coli GFP toxicity biosensor in real water samples toxicity evaluation.
American Psychological Association (APA)
Ooi, Lia& Heng, Lee Yook& Ahmad, Asmat. 2015. Toxicity Biosensor for Sodium Dodecyl Sulfate Using Immobilized Green Fluorescent Protein Expressing Escherichia coli. Journal of Sensors،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1070200
Modern Language Association (MLA)
Ooi, Lia…[et al.]. Toxicity Biosensor for Sodium Dodecyl Sulfate Using Immobilized Green Fluorescent Protein Expressing Escherichia coli. Journal of Sensors No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1070200
American Medical Association (AMA)
Ooi, Lia& Heng, Lee Yook& Ahmad, Asmat. Toxicity Biosensor for Sodium Dodecyl Sulfate Using Immobilized Green Fluorescent Protein Expressing Escherichia coli. Journal of Sensors. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1070200
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1070200