Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif
Joint Authors
Štěpánek, Josef
Římal, Václav
Socha, Ondřej
Štěpánková, Helena
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Effect of cytosine methylation on DNA duplexes was studied by using a model system of three self-complementary DNA octamers containing central CpG motif surrounded by a couple of AT base pairs, CAACGTTG, CATCGATG, and CTTCGAAG, and their analogues with the central cytosine methylated at C5 position.
Temperature dependences of 1H NMR, UV absorption, and Raman scattering spectra measured for aqueous solutions at concentrations of different orders of magnitude were subjected to a joint analysis that allowed an accurate determination of the enthalpy and entropy of duplex formation.
It was revealed that the changes of the enthalpy and entropy contributions are strongly dependent on the base composition in the vicinity of the CpG motif.
American Psychological Association (APA)
Římal, Václav& Socha, Ondřej& Štěpánek, Josef& Štěpánková, Helena. 2015. Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif. Journal of Spectroscopy،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1070352
Modern Language Association (MLA)
Římal, Václav…[et al.]. Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif. Journal of Spectroscopy No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1070352
American Medical Association (AMA)
Římal, Václav& Socha, Ondřej& Štěpánek, Josef& Štěpánková, Helena. Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif. Journal of Spectroscopy. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1070352
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1070352