LUBAC Formation Is Impaired in the Livers of Mice with MCD-Dependent Nonalcoholic Steatohepatitis
Joint Authors
Matsunaga, Yasuka
Nakatsu, Yusuke
Fukushima, Toshiaki
Okubo, Hirofumi
Iwashita, Misaki
Sakoda, Hideyuki
Fujishiro, Midori
Yamamotoya, Takeshi
Kushiyama, Akifumi
Takahashi, Shin-ichiro
Tsuchiya, Yoshihiro
Kamata, Hideaki
Tokunaga, Fuminori
Iwai, Kazuhiro
Asano, Tomoichiro
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Nonalcoholic steatohepatitis (NASH) is a disorder characterized by hepatic lipid accumulation followed by the inflammation-induced death of hepatocytes and fibrosis.
In this process, oxidative stress contributes to the induction of several inflammatory cytokines including TNF-α and IL-1β in macrophages, while, in hepatocytes, NF-κB reportedly induces the expressions of cell survival genes for protection from apoptosis.
Recently, it was reported that the new ubiquitin ligase complex termed linear ubiquitin chain assembly complex (LUBAC), composed of SHARPIN (SHANK-associated RH domain-interacting protein), HOIL-1L (longer isoform of heme-oxidized iron-regulatory protein 2 ubiquitin ligase-1), and HOIP (HOIL-1L interacting protein), forms linear ubiquitin on NF-κB essential modulator (NEMO) and thereby induces NF-κB pathway activation.
In this study, we demonstrated the formation of LUBAC to be impaired in the livers of NASH rodent models produced by methionine and choline deficient (MCD) diet feeding, first by either gel filtration or Blue Native-PAGE, with subsequent confirmation by western blotting.
The reduction of LUBAC is likely to be attributable to markedly reduced expression of SHARPIN, one of its components.
Thus, impaired LUBAC formation, which would result in insufficient NF-κB activation, may be one of the molecular mechanisms underlying the enhanced apoptotic response of hepatocytes in MCD diet-induced NASH livers.
American Psychological Association (APA)
Matsunaga, Yasuka& Nakatsu, Yusuke& Fukushima, Toshiaki& Okubo, Hirofumi& Iwashita, Misaki& Sakoda, Hideyuki…[et al.]. 2015. LUBAC Formation Is Impaired in the Livers of Mice with MCD-Dependent Nonalcoholic Steatohepatitis. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072123
Modern Language Association (MLA)
Matsunaga, Yasuka…[et al.]. LUBAC Formation Is Impaired in the Livers of Mice with MCD-Dependent Nonalcoholic Steatohepatitis. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072123
American Medical Association (AMA)
Matsunaga, Yasuka& Nakatsu, Yusuke& Fukushima, Toshiaki& Okubo, Hirofumi& Iwashita, Misaki& Sakoda, Hideyuki…[et al.]. LUBAC Formation Is Impaired in the Livers of Mice with MCD-Dependent Nonalcoholic Steatohepatitis. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072123
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072123