Interplay between Intravitreal RvD1 and Local Endogenous Sirtuin-1 in the Protection from Endotoxin-Induced Uveitis in Rats
Joint Authors
Testa, Francesco
Di Filippo, Clara
Franca, Ferraraccio
Maisto, R.
Trotta, M. C.
Ferraro, B.
Gesualdo, C.
Rossi, S.
Accardo, M.
D'Amico, Michele
Simonelli, Francesca
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Rat endotoxin-induced uveitis (EIU) is a well-established model of human uveitis.
In this model, intravitreal injection of resolvin D1 (RvD1, 10–100–1000 ng/kg) 1 hour after subcutaneous treatment of Sprague-Dawley rats with lipopolysaccharide (LPS, 200 μg/rat) significantly prevented the development of uveitis into the eye.
RvD1 dose-dependently increased the expression of sirtuin-1 (SIRT1) within the eye, while it decreased the expression of acetyl-p53 and acetyl-FOXO1.
These effects were accompanied by local downregulation of some microRNAs related to the expression and activity of SIRT1.
These were miR-195-5p, miR-200a-3p, miR-34a-5p, and miR-145-5p.
An increase of manganese superoxide dismutase and decrease of caspase 3 were evident after RvD1 treatment.
In another set of experiments, the protective effects of RvD1 (1000 ng/kg) were partly abolished by the pretreatment of the rats with EX527 (10 mg/kg/day, i.p.), a specific inhibitor of SIRT1 activity, for 7 days prior to the induction of EIU in rats.
Similarly, the effects of RvD1 (1000 ng/kg) on the SIRT1 protein expression were abolished by Boc2, N-t-butoxycarbonyl-PLPLP, a specific formyl-peptide receptor type 2/lipoxin A receptor antagonist.
Therefore, an interplay of the SIRT1 activity on the RvD1 mediated resolution of EIU is argued.
American Psychological Association (APA)
Rossi, S.& Di Filippo, Clara& Gesualdo, C.& Testa, Francesco& Trotta, M. C.& Maisto, R.…[et al.]. 2015. Interplay between Intravitreal RvD1 and Local Endogenous Sirtuin-1 in the Protection from Endotoxin-Induced Uveitis in Rats. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072125
Modern Language Association (MLA)
Rossi, S.…[et al.]. Interplay between Intravitreal RvD1 and Local Endogenous Sirtuin-1 in the Protection from Endotoxin-Induced Uveitis in Rats. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072125
American Medical Association (AMA)
Rossi, S.& Di Filippo, Clara& Gesualdo, C.& Testa, Francesco& Trotta, M. C.& Maisto, R.…[et al.]. Interplay between Intravitreal RvD1 and Local Endogenous Sirtuin-1 in the Protection from Endotoxin-Induced Uveitis in Rats. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072125
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072125