Protection from Endotoxic Uveitis by Intravitreal Resolvin D1: Involvement of Lymphocytes, miRNAs, Ubiquitin-Proteasome, and M1M2 Macrophages
Joint Authors
Potenza, Nicoletta
Di Filippo, Clara
Franca, Ferraraccio
Zippo, M. V.
Maisto, R.
Gesualdo, C.
Rossi, S.
Trotta, M. C.
Russo, Aniello
D'Amico, Michele
Simonelli, Francesca
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
This study investigated the protective effects of intravitreal Resolvin D1 (RvD1) against LPS-induced rat endotoxic uveitis (EIU).
RvD1 was administered into the right eye at a single injection of 5 μL volume containing 10–100–1000 ng/kg RvD1 1 h post-LPS injection (200 μg, Salmonella minnesota) into thefootpad of Sprague-Dawley rats.
24 h later, the eye was enucleated and examined for clinical, biochemical, and immunohistochemical evaluations.
RvD1 significantly and dose-dependently decreased the clinical score attributed to EIU, starting from the dose of 10 ng/kg and further decreased by 100 and 1000 ng/kg.
These effects were accompanied by changes in four important determinants of the immune-inflammatory response within the eye: (i) the B and T lymphocytes, (ii) the miRNAs pattern, (iii) the ubiquitin-proteasome system (UPS), and (iv) the M1/M2 macrophage phenotype.
LPS+RvD1 treated rats showed reduced presence of B and T lymphocytes and upregulation of miR-200c-3p, miR 203a-3p, miR 29b-3p, and miR 21-5p into the eye compared to the LPS alone.
This was paralleled by decreases of the ubiquitin, 20S and 26S proteasome subunits, reduced presence of macrophage M1, and increased presence of macrophage M2 in the ocular tissues.
Accordingly, the levels of the cytokine TNF-α, the chemokines MIP1-α and NF-κB were reduced.
American Psychological Association (APA)
Rossi, S.& Di Filippo, Clara& Gesualdo, C.& Potenza, Nicoletta& Russo, Aniello& Trotta, M. C.…[et al.]. 2015. Protection from Endotoxic Uveitis by Intravitreal Resolvin D1: Involvement of Lymphocytes, miRNAs, Ubiquitin-Proteasome, and M1M2 Macrophages. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1072157
Modern Language Association (MLA)
Rossi, S.…[et al.]. Protection from Endotoxic Uveitis by Intravitreal Resolvin D1: Involvement of Lymphocytes, miRNAs, Ubiquitin-Proteasome, and M1M2 Macrophages. Mediators of Inflammation No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1072157
American Medical Association (AMA)
Rossi, S.& Di Filippo, Clara& Gesualdo, C.& Potenza, Nicoletta& Russo, Aniello& Trotta, M. C.…[et al.]. Protection from Endotoxic Uveitis by Intravitreal Resolvin D1: Involvement of Lymphocytes, miRNAs, Ubiquitin-Proteasome, and M1M2 Macrophages. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1072157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072157