High-Salt Enhances the Inflammatory Response by Retina Pigment Epithelium Cells following Lipopolysaccharide Stimulation
Joint Authors
Wang, Chaokui
Ye, Zi
Kijlstra, Aize
Yang, Peizeng
Zhang, Dike
Cao, Shuang
Deng, Bolin
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
High-salt has been shown to play a role in the pathogenesis of autoimmune disease.
In this study, we investigated the effect of high-salt on the production of inflammatory mediators by ARPE-19 cells and the possible mechanisms involved.
ARPE-19 cells were cultured with LPS in DMEM to which extra NaCl had been added (20 mM and 40 mM).
NaCl had no influence on the apoptosis and proliferation of ARPE-19.
Addition of 40 mM NaCl significantly induced IL-6 and MCP-1 production but had no effect on IL-8 secretion.
High mannitol, as an osmotic stress control, did not affect the secretion of inflammatory mediators by ARPE-19 cells indicating that the effect was not mediated by osmolarity.
Coculture of ARPE-19 cells with NaCl resulted in significant increases in the phosphorylation of p38 MAPK, Akt, and NF-κB and an upregulation of the transcription factors NFAT5 and SGK1.
High-salt significantly promotes IL-6 and MCP-1 production by ARPE-19 cells and is associated with activation of the p38 MAPK, Akt, and NF-κB pathway and NFAT-SGK1 pathways.
American Psychological Association (APA)
Zhang, Dike& Wang, Chaokui& Cao, Shuang& Ye, Zi& Deng, Bolin& Kijlstra, Aize…[et al.]. 2015. High-Salt Enhances the Inflammatory Response by Retina Pigment Epithelium Cells following Lipopolysaccharide Stimulation. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072180
Modern Language Association (MLA)
Zhang, Dike…[et al.]. High-Salt Enhances the Inflammatory Response by Retina Pigment Epithelium Cells following Lipopolysaccharide Stimulation. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072180
American Medical Association (AMA)
Zhang, Dike& Wang, Chaokui& Cao, Shuang& Ye, Zi& Deng, Bolin& Kijlstra, Aize…[et al.]. High-Salt Enhances the Inflammatory Response by Retina Pigment Epithelium Cells following Lipopolysaccharide Stimulation. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072180
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072180