Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes
Joint Authors
Dias-Melicio, Luciane Alarcão
Fernandes, Reginaldo Keller
Rodrigues, Daniela Ramos
Golim, Marjorie Assis
Soares, Angela Maria Victoriano Campos
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Interleukin-18 is a proinflammatory cytokine belonging to the interleukin-1 family of cytokines.
This cytokine exerts many unique biological and immunological effects.
To explore the role of IL-18 in inflammatory innate immune responses, we investigated its impact on expression of two toll-like receptors (TLR2 and TLR4) and mannose receptor (MR) by human peripheral blood monocytes and its effect on TNF-α, IL-12, IL-15, and IL-10 production.
Monocytes from healthy donors were stimulated or not with IL-18 for 18 h, and then the TLR2, TLR4, and MR expression and intracellular TNF-α, IL-12, and IL-10 production were assessed by flow cytometry and the levels of TNF-α, IL-12, IL-15, and IL-10 in culture supernatants were measured by ELISA.
IL-18 treatment was able to increase TLR4 and MR expression by monocytes.
The production of TNF-α and IL-10 was also increased by cytokine treatment.
However, IL-18 was unable to induce neither IL-12 nor IL-15 production by these cells.
Taken together, these results show an important role of IL-18 on the early phase of inflammatory response by promoting the expression of some pattern recognition receptors (PRRs) that are important during the microbe recognition phase and by inducing some important cytokines such as TNF-α and IL-10.
American Psychological Association (APA)
Dias-Melicio, Luciane Alarcão& Fernandes, Reginaldo Keller& Rodrigues, Daniela Ramos& Golim, Marjorie Assis& Soares, Angela Maria Victoriano Campos. 2015. Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072195
Modern Language Association (MLA)
Dias-Melicio, Luciane Alarcão…[et al.]. Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes. Mediators of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1072195
American Medical Association (AMA)
Dias-Melicio, Luciane Alarcão& Fernandes, Reginaldo Keller& Rodrigues, Daniela Ramos& Golim, Marjorie Assis& Soares, Angela Maria Victoriano Campos. Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072195
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072195