![](/images/graphics-bg.png)
Anti-Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study
Joint Authors
Monfrecola, Giuseppe
Di Costanzo, Luisa
Balato, Anna
Lembo, Serena
Di Caprio, Roberta
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Doxycycline is used to treat infective diseases because of its broadspectrum efficacy.
High dose administration (100 or 200 mg/day) is often responsible for development of bacterial resistances and endogenous flora alterations, whereas low doses (20–40 mg/day) do not alter bacteria susceptibility to antibiotics and exert anti-inflammatory activities.
In this study, we wanted to assess the efficacy of both low and high doxycycline doses in modulating IL-8, TNF-α, and IL-6 gene expression in HaCaT cells stimulated with LPS.
Three experimental settings were used, differing in the timing of doxycycline treatment in respect to the insult induced by LPS: pretreatment, concomitant, and posttreatment.
Low doses were more effective than high doses in modulating gene expression of LPS-induced proinflammatory cytokines (IL-8, TNF-α, and IL-6), when added before (pretreatment) or after (posttreatment) LPS stimulation.
This effect was not appreciated when LPS and doxycycline were simultaneously added to cell cultures: in this case high doses were more effective.
In conclusion, our in vitro study suggests that low doxycycline doses could be safely used in chronic or acute skin diseases in which the inflammatory process, either constantly in progress or periodically recurring, has to be prevented or controlled.
American Psychological Association (APA)
Di Caprio, Roberta& Lembo, Serena& Di Costanzo, Luisa& Balato, Anna& Monfrecola, Giuseppe. 2015. Anti-Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072252
Modern Language Association (MLA)
Di Caprio, Roberta…[et al.]. Anti-Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072252
American Medical Association (AMA)
Di Caprio, Roberta& Lembo, Serena& Di Costanzo, Luisa& Balato, Anna& Monfrecola, Giuseppe. Anti-Inflammatory Properties of Low and High Doxycycline Doses: An In Vitro Study. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072252
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072252