Effects of Intravenous Injection of Porphyromonas gingivalis on Rabbit Inflammatory Immune Response and Atherosclerosis
Joint Authors
Lin, Gengbing
Chen, Shuai
Lei, Lang
You, Xiaoqing
Huang, Min
Luo, Lan
Li, Yanfen
Zhao, Xin
Yan, Fuhua
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The effects of intravenous injection of Porphyromonas gingivalis (Pg) on rabbit inflammatory immune response and atherosclerosis were evaluated by establishing a microamount Pg bacteremia model combined with high-fat diet.
Twenty-four New Zealand rabbits were randomly divided into Groups A-D n = 6 .
After 14 weeks, levels of inflammatory factors (C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1)) in peripheral blood were detected by ELISA.
The aorta was subjected to HE staining.
Local aortic expressions of toll-like receptor-2 (TLR-2), TLR-4, TNF-α, CRP, IL-6, matrix metallopeptidase-9, and MCP-1 were detected by real-time PCR, and those of nuclear factor-κB (NF-κB) p65, phospho-p38 mitogen-activated protein kinase (MAPK), and phospho-c-Jun N-terminal kinase (JNK) proteins were detected by Western blot.
Intravenous injection of Pg to the bloodstream alone induced atherosclerotic changes and significantly increased systemic and local aortic expressions of inflammatory factors, NF-κB p65, phospho-p38-MAPK, and JNK, especially in Group D.
Injection of microamount Pg induced inflammatory immune response and accelerated atherosclerosis, in which the NF-κB p65, p38-MAPK, and JNK signaling pathways played important roles.
Intravenous injection of Pg is not the same as Pg from human periodontitis entering the blood stream.
Therefore, our results cannot be extrapolated to human periodontitis.
American Psychological Association (APA)
Lin, Gengbing& Chen, Shuai& Lei, Lang& You, Xiaoqing& Huang, Min& Luo, Lan…[et al.]. 2015. Effects of Intravenous Injection of Porphyromonas gingivalis on Rabbit Inflammatory Immune Response and Atherosclerosis. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072271
Modern Language Association (MLA)
Lin, Gengbing…[et al.]. Effects of Intravenous Injection of Porphyromonas gingivalis on Rabbit Inflammatory Immune Response and Atherosclerosis. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072271
American Medical Association (AMA)
Lin, Gengbing& Chen, Shuai& Lei, Lang& You, Xiaoqing& Huang, Min& Luo, Lan…[et al.]. Effects of Intravenous Injection of Porphyromonas gingivalis on Rabbit Inflammatory Immune Response and Atherosclerosis. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072271