Kaurenoic Acid Possesses Leishmanicidal Activity by Triggering a NLRP12IL-1βcNOSNO Pathway
Joint Authors
da Silva, Suelen Santos
Verri, Waldiceu A.
Cecchini, Rubens
Panis, Carolina
Ambrosio, Sergio R.
Macri, Juliana Aparecida
Kawakami, Natalia Yoshie
Hayashida, Thiago Hideki
Madeira, Tiago Bervelieri
Acquaro, Vinicius Ricardo
Nixdorf, Suzana Lucy
Pizzatti, Luciana
Arakawa, Nilton Syogo
Miranda, Milena Menegazzo
Conchon-Costa, Ivete
Pavanelli, Wander Rogério
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Leishmania amazonensis (L.
amazonensis) infection can cause severe local and diffuse injuries in humans, a condition clinically known as American cutaneous leishmaniasis (ACL).
Currently, the therapeutic approach for ACL is based on Glucantime, which shows high toxicity and poor effectiveness.
Therefore, ACL remains a neglected disease with limited options for treatment.
Herein, the in vitro antiprotozoal effect and mechanisms of the diterpene kaurenoic acid [ent-kaur-16-en-19-oic acid] (KA) against L.
amazonensis were investigated.
KA exhibited a direct antileishmanial effect on L.
amazonensis promastigotes.
Importantly, KA also reduced the intracellular number of amastigote forms and percentage of infected peritoneal macrophages of BALB/c mice.
Mechanistically, KA treatment reestablished the production of nitric oxide (NO) in a constitutive NO synthase- (cNOS-) dependent manner, subverting the NO-depleting escape mechanism of L.
amazonensis.
Furthermore, KA induced increased production of IL-1β and expression of the inflammasome-activating component NLRP12.
These findings demonstrate the leishmanicidal capability of KA against L.
amazonensis in macrophage culture by triggering a NLRP12/IL-1β/cNOS/NO mechanism.
American Psychological Association (APA)
Miranda, Milena Menegazzo& Panis, Carolina& da Silva, Suelen Santos& Macri, Juliana Aparecida& Kawakami, Natalia Yoshie& Hayashida, Thiago Hideki…[et al.]. 2015. Kaurenoic Acid Possesses Leishmanicidal Activity by Triggering a NLRP12IL-1βcNOSNO Pathway. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072302
Modern Language Association (MLA)
Miranda, Milena Menegazzo…[et al.]. Kaurenoic Acid Possesses Leishmanicidal Activity by Triggering a NLRP12IL-1βcNOSNO Pathway. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072302
American Medical Association (AMA)
Miranda, Milena Menegazzo& Panis, Carolina& da Silva, Suelen Santos& Macri, Juliana Aparecida& Kawakami, Natalia Yoshie& Hayashida, Thiago Hideki…[et al.]. Kaurenoic Acid Possesses Leishmanicidal Activity by Triggering a NLRP12IL-1βcNOSNO Pathway. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072302
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072302