A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation
Joint Authors
Du, Lei
Luo, Shuhua
Tang, Menglin
Gong, Lina
Xu, Jin
Chen, Youwen
Wang, Yabo
An, Qi
Dian, Ke
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Extracorporeal circulation (ECC) is necessary for conventional cardiac surgery and life support, but it often triggers systemic inflammation that can significantly damage tissue.
Studies of ECC have been limited to large animals because of the complexity of the surgical procedures involved, which has hampered detailed understanding of ECC-induced injury.
Here we describe a minimally invasive mouse model of ECC that may allow more extensive mechanistic studies.
The right carotid artery and external jugular vein of anesthetized adult male C57BL/6 mice were cannulated to allow blood flow through a 1/32-inch external tube.
All animals ( n = 2 0 ) survived 30 min ECC and subsequent 60 min observation.
Blood analysis after ECC showed significant increases in levels of tumor necrosis factor α, interleukin-6, and neutrophil elastase in plasma, lung, and renal tissues, as well as increases in plasma creatinine and cystatin C and decreases in the oxygenation index.
Histopathology showed that ECC induced the expected lung inflammation, which included alveolar congestion, hemorrhage, neutrophil infiltration, and alveolar wall thickening; in renal tissue, ECC induced intracytoplasmic vacuolization, acute tubular necrosis, and epithelial swelling.
Our results suggest that this novel, minimally invasive mouse model can recapitulate many of the clinical features of ECC-induced systemic inflammatory response and organ injury.
American Psychological Association (APA)
Luo, Shuhua& Tang, Menglin& Du, Lei& Gong, Lina& Xu, Jin& Chen, Youwen…[et al.]. 2015. A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072312
Modern Language Association (MLA)
Luo, Shuhua…[et al.]. A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation. Mediators of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1072312
American Medical Association (AMA)
Luo, Shuhua& Tang, Menglin& Du, Lei& Gong, Lina& Xu, Jin& Chen, Youwen…[et al.]. A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072312
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072312