Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation

Joint Authors

Sperandio, Felipe Fornias
Fernandes, Gisele Pesquero
Mendes, Ana Carolina Silvério Cerqueira
Bani, Giulia Maria de Alencar Castro
Calich, Vera Lucia Garcia
Burger, Eva

Source

Mediators of Inflammation

Issue

Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2015-12-24

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Diseases

Abstract EN

Paracoccidioidomycosis (PCM) is a systemic fungal infection, endemic in Brazil, that leads to severe morbidity and even mortality if not correctly treated.

Patients may respond differently to PCM depending on the pattern of the acquired immune response developed.

The onset of protective immune response is notably mediated by neutrophils (PMN) that play an important role through directly killing the fungi and also by interacting with other cell types to modulate the acquired protective immune response that may follow.

In that way, this study aimed to present and compare different experimental models of PCM (intraperitoneal and subcutaneous) regarding PMN production and maturation inside femoral bone marrow and also PMN infiltration in peritoneal and subcutaneous exudates of resistant and susceptible mice.

We also assessed the fungal colony forming units and the levels of soluble inflammatory mediators (LTB4, KC, IFN-γ, GM-CSF, and IL-10) inside subcutaneous air-pouches to compare the efficiency of the PMN present at this site in relation to the two main neutrophil functions: initial lysis of the invading pathogen and modulation of the acquired immune response.

P.

brasiliensis inoculated intraperitoneally was able to disseminate to the bone marrow of susceptible mice, causing a more marked alteration of PMN production and maturation than that observed after resistant mice infection by the same route.

Subcutaneous air-pouch inoculation of P.

brasiliensis elicited a controlled and limited infection that produced a PMN-rich exudate, thus favoring the study of the interaction between the fungus and the neutrophils.

Susceptible mice produced higher numbers of PMN; however, these cells were less effective in killing the fungi.

Inflammatory cytokines were more pronounced in resistant mice, which supports their PCM raised resistance.

American Psychological Association (APA)

Sperandio, Felipe Fornias& Fernandes, Gisele Pesquero& Mendes, Ana Carolina Silvério Cerqueira& Bani, Giulia Maria de Alencar Castro& Calich, Vera Lucia Garcia& Burger, Eva. 2015. Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072326

Modern Language Association (MLA)

Sperandio, Felipe Fornias…[et al.]. Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072326

American Medical Association (AMA)

Sperandio, Felipe Fornias& Fernandes, Gisele Pesquero& Mendes, Ana Carolina Silvério Cerqueira& Bani, Giulia Maria de Alencar Castro& Calich, Vera Lucia Garcia& Burger, Eva. Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072326

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1072326