Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and Suppresses Expression of Inflammatory Mediators in the Rat Model of Brain Injury
Joint Authors
Savic, Danijela
Stojiljkovic, Mirjana
Pekovic, Sanja
Lavrnja, Irena
Parabucki, Ana
Brkic, Predrag
Jovanovic, Tomislav
Dacic, Sanja
Pantic, Igor
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-20
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The exact mechanisms by which treatment with hyperbaric oxygen (HBOT) exerts its beneficial effects on recovery after brain injury are still unrevealed.
Therefore, in this study we investigated the influence of repetitive HBOT on the reactive astrogliosis and expression of mediators of inflammation after cortical stab injury (CSI).
CSI was performed on male Wistar rats, divided into control, sham, and lesioned groups with appropriate HBO.
The HBOT protocol was as follows: 10 minutes of slow compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10 minutes of slow decompression, once a day for 10 consecutive days.
Data obtained using real-time polymerase chain reaction, Western blot, and immunohistochemical and immunofluorescence analyses revealed that repetitive HBOT applied after the CSI attenuates reactive astrogliosis and glial scarring, and reduces expression of GFAP (glial fibrillary acidic protein), vimentin, and ICAM-1 (intercellular adhesion molecule-1) both at gene and tissue levels.
In addition, HBOT prevents expression of CD40 and its ligand CD40L on microglia, neutrophils, cortical neurons, and reactive astrocytes.
Accordingly, repetitive HBOT, by prevention of glial scarring and limiting of expression of inflammatory mediators, supports formation of more permissive environment for repair and regeneration.
American Psychological Association (APA)
Lavrnja, Irena& Parabucki, Ana& Brkic, Predrag& Jovanovic, Tomislav& Dacic, Sanja& Savic, Danijela…[et al.]. 2015. Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and Suppresses Expression of Inflammatory Mediators in the Rat Model of Brain Injury. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1072389
Modern Language Association (MLA)
Lavrnja, Irena…[et al.]. Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and Suppresses Expression of Inflammatory Mediators in the Rat Model of Brain Injury. Mediators of Inflammation No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1072389
American Medical Association (AMA)
Lavrnja, Irena& Parabucki, Ana& Brkic, Predrag& Jovanovic, Tomislav& Dacic, Sanja& Savic, Danijela…[et al.]. Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and Suppresses Expression of Inflammatory Mediators in the Rat Model of Brain Injury. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1072389
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072389