Postmitotic Expression of SOD1G93A Gene Affects the Identity of Myogenic Cells and Inhibits Myoblasts Differentiation
Joint Authors
Musarò, Antonio
Martini, Martina
Dobrowolny, Gabriella
Aucello, Michela
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-28
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
To determine the role of mutant SOD1 gene (SOD1G93A) on muscle cell differentiation, we derived C2C12 muscle cell lines carrying a stably transfected SOD1G93A gene under the control of a myosin light chain (MLC) promoter-enhancer cassette.
Expression of MLC/SOD1G93A in C2C12 cells resulted in dramatic inhibition of myoblast differentiation.
Transfected SOD1G93A gene expression in postmitotic skeletal myocytes downregulated the expression of relevant markers of committed and differentiated myoblasts such as MyoD, Myogenin, MRF4, and the muscle specific miRNA expression.
The inhibitory effects of SOD1G93A gene on myogenic program perturbed Akt/p70 and MAPK signaling pathways which promote differentiation cascade.
Of note, the inhibition of the myogenic program, by transfected SOD1G93A gene expression, impinged also the identity of myogenic cells.
Expression of MLC/SOD1G93A in C2C12 myogenic cells promoted a fibro-adipogenic progenitors (FAPs) phenotype, upregulating HDAC4 protein and preventing the myogenic commitment complex BAF60C-SWI/SNF.
We thus identified potential molecular mediators of the inhibitory effects of SOD1G93A on myogenic program and disclosed potential signaling, activated by SOD1G93A, that affect the identity of the myogenic cell population.
American Psychological Association (APA)
Martini, Martina& Dobrowolny, Gabriella& Aucello, Michela& Musarò, Antonio. 2015. Postmitotic Expression of SOD1G93A Gene Affects the Identity of Myogenic Cells and Inhibits Myoblasts Differentiation. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1072412
Modern Language Association (MLA)
Martini, Martina…[et al.]. Postmitotic Expression of SOD1G93A Gene Affects the Identity of Myogenic Cells and Inhibits Myoblasts Differentiation. Mediators of Inflammation No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1072412
American Medical Association (AMA)
Martini, Martina& Dobrowolny, Gabriella& Aucello, Michela& Musarò, Antonio. Postmitotic Expression of SOD1G93A Gene Affects the Identity of Myogenic Cells and Inhibits Myoblasts Differentiation. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1072412
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072412