Human Leucocyte Antigen-G (HLA-G)‎ and Its Murine Functional Homolog Qa2 in the Trypanosoma cruzi Infection

Abstract EN

Genetic susceptibility factors, parasite strain, and an adequate modulation of the immune system seem to be crucial for disease progression after Trypanosoma cruzi infection.

HLA-G and its murine functional homolog Qa2 have well-recognized immunomodulatory properties.

We evaluated the HLA-G 3′ untranslated region (3′UTR) polymorphic sites (associated with mRNA stability and target for microRNA binding) and HLA-G tissue expression (heart, colon, and esophagus) in patients presenting Chagas disease, stratified according to the major clinical variants.

Further, we investigated the transcriptional levels of Qa2 and other pro- and anti-inflammatory genes in affected mouse tissues during T.

cruzi experimental acute and early chronic infection induced by the CL strain.

Chagas disease patients exhibited differential HLA-G 3′UTR susceptibility allele/genotype/haplotype patterns, according to the major clinical variant (digestive/cardiac/mixed/indeterminate).

HLA-G constitutive expression on cardiac muscle and colonic cells was decreased in Chagasic tissues; however, no difference was observed for Chagasic and non-Chagasic esophagus tissues.

The transcriptional levels of Qa2 and other anti and proinflammatory (CTLA-4, PDCD1, IL-10, INF-γ, and NOS-2) genes were induced only during the acute T.

cruzi infection in BALB/c and C57BL/6 mice.

We present several lines of evidence indicating the role of immunomodulatory genes and molecules in human and experimental T.

cruzi infection.