Mechanisms of Thymoquinone Hepatorenal Protection in Methotrexate-Induced Toxicity in Rats
Joint Authors
Abdalla, Ahlam M.
Hamouda, Azza H.
Alhaider, Ibrahim A.
Morsy, Mohamed A.
El-Sheikh, Azza A. K.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
To investigate mechanisms by which thymoquinone (TQ) can prevent methotrexate- (MTX-) induced hepatorenal toxicity, TQ (10 mg/kg) was administered orally for 10 days.
In independent rat groups, MTX hepatorenal toxicity was induced via 20 mg/kg i.p.
at the end of day 3 of experiment, with or without TQ.
MTX caused deterioration in kidney and liver function, namely, blood urea nitrogen, creatinine, alanine aminotransferase, and aspartate aminotransferase.
MTX also caused distortion in renal and hepatic histology, with significant oxidative stress, manifested by decrease in reduced glutathione and catalase, as well as increase in malondialdehyde levels.
In addition, MTX caused nitrosative stress manifested by increased nitric oxide, with upregulation of inducible nitric oxide synthase.
Furthermore, MTX caused hepatorenal inflammatory effects as shown by increased tumor necrosis factor-α, besides upregulation of necrosis factor-κB and cyclooxygenase-2 expressions.
MTX also caused apoptotic effect, as it upregulated caspase 3 in liver and kidney.
Using TQ concurrently with MTX restored kidney and liver functions, as well as their normal histology.
TQ also reversed oxidative and nitrosative stress, as well as inflammatory and apoptotic signs caused by MTX alone.
Thus, TQ may be beneficial adjuvant that confers hepatorenal protection to MTX toxicity via antioxidant, antinitrosative, anti-inflammatory, and antiapoptotic mechanisms.
American Psychological Association (APA)
El-Sheikh, Azza A. K.& Morsy, Mohamed A.& Abdalla, Ahlam M.& Hamouda, Azza H.& Alhaider, Ibrahim A.. 2015. Mechanisms of Thymoquinone Hepatorenal Protection in Methotrexate-Induced Toxicity in Rats. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1072616
Modern Language Association (MLA)
El-Sheikh, Azza A. K.…[et al.]. Mechanisms of Thymoquinone Hepatorenal Protection in Methotrexate-Induced Toxicity in Rats. Mediators of Inflammation No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1072616
American Medical Association (AMA)
El-Sheikh, Azza A. K.& Morsy, Mohamed A.& Abdalla, Ahlam M.& Hamouda, Azza H.& Alhaider, Ibrahim A.. Mechanisms of Thymoquinone Hepatorenal Protection in Methotrexate-Induced Toxicity in Rats. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1072616
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072616