![](/images/graphics-bg.png)
The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model
Joint Authors
Ruchholtz, Steffen
Schwarting, Tim
Pretzsch, Sebastian
Debus, Florian
Lechler, Philipp
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The effects of cyclooxygenase (COX) inhibition following the reconstruction of the anterior cruciate ligament remain unclear.
We examined the effects of selective COX-2 and nonselective COX inhibition on bone-tendon integration in an in vitro model.
We measured the dose-dependent effects of ibuprofen and parecoxib on the viability of lipopolysaccharide- (LPS-) stimulated and unstimulated mouse MC3T3-E1 and 3T3 cells, the influence on gene expression at the osteoblast, interface, and fibroblast regions measured by quantitative PCR, and cellular outgrowth assessed on histological sections.
Ibuprofen led to a dose-dependent suppression of MC3T3 cell viability, while parecoxib reduced the viability of 3T3 cultures.
Exposure to ibuprofen significantly suppressed expression of Alpl (P < 0.01), Bglap (P < 0.001), and Runx2 (P < 0.01), and although parecoxib reduced expression of Alpl (P < 0.001), Fmod (P < 0.001), and Runx2 (P < 0.01), the expression of Bglap was increased (P < 0.01).
Microscopic analysis showed a reduction in cellular outgrowth in LPS-stimulated cultures following exposure to ibuprofen and parecoxib.
Nonselective COX inhibition and the specific inhibition of COX-2 led to region-specific reductions in markers of calcification and cell viability.
We suggest further in vitro and in vivo studies examining the biologic and biomechanical effects of selective and nonselective COX inhibition.
American Psychological Association (APA)
Schwarting, Tim& Pretzsch, Sebastian& Debus, Florian& Ruchholtz, Steffen& Lechler, Philipp. 2015. The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072640
Modern Language Association (MLA)
Schwarting, Tim…[et al.]. The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072640
American Medical Association (AMA)
Schwarting, Tim& Pretzsch, Sebastian& Debus, Florian& Ruchholtz, Steffen& Lechler, Philipp. The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072640
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072640