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Bleomycin-Treated Chimeric Thy1-Deficient Mice with Thy1-Deficient Myofibroblasts and Thy-Positive Lymphocytes Resolve Inflammation without Affecting the Fibrotic Response
Joint Authors
Cohen, Pazit Y.
Breuer, Raphael
Zisman, Philip
Wallach-Dayan, Shulamit B.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-02
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Lung fibrosis is characterized by abnormal accumulation of fibroblasts in the interstitium of the alveolar space.
Two populations of myofibroblasts, distinguished by Thy1 expression, are detected in human and murine lungs.
Accumulation of Thy1-negative (Thy1−) myofibroblasts was shown in the lungs of humans with idiopathic pulmonary fibrosis (IPF) and of bleomycin-treated mice.
We aimed to identify genetic changes in lung myofibroblasts following Thy1 crosslinking and assess the impact of specific lung myofibroblast Thy1-deficiency, in vivo, in bleomycin-injured mouse lungs.
Thy1 increased in mouse lung lymphocytes following bleomycin injury but decreased in myofibroblasts when fibrosis was at the highest point (14 days), as assessed by immunohistochemistry.
Using gene chip analysis, we detected that myofibroblast Thy1 crosslinking mediates downregulation of genes promoting cell proliferation, survival, and differentiation, and reduces production of extracellular matrix (ECM) components, while concurrently mediating the upregulation of genes known to foster inflammation and immunological functions.
Chimeric Thy1-deficient mice with Thy1+ lymphocytes and Thy1− myofibroblasts showed fibrosis similar to wild-type mice and an increased number of CD4/CD25 regulatory T cells, with a concomitant decrease in inflammation.
Lung myofibroblasts downregulate Thy1 expression to increase their proliferation but to diminish the in vivo inflammatory milieu.
Inflammation is not essential for evolution of fibrosis as was previously stated.
American Psychological Association (APA)
Cohen, Pazit Y.& Breuer, Raphael& Zisman, Philip& Wallach-Dayan, Shulamit B.. 2015. Bleomycin-Treated Chimeric Thy1-Deficient Mice with Thy1-Deficient Myofibroblasts and Thy-Positive Lymphocytes Resolve Inflammation without Affecting the Fibrotic Response. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1072649
Modern Language Association (MLA)
Cohen, Pazit Y.…[et al.]. Bleomycin-Treated Chimeric Thy1-Deficient Mice with Thy1-Deficient Myofibroblasts and Thy-Positive Lymphocytes Resolve Inflammation without Affecting the Fibrotic Response. Mediators of Inflammation No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1072649
American Medical Association (AMA)
Cohen, Pazit Y.& Breuer, Raphael& Zisman, Philip& Wallach-Dayan, Shulamit B.. Bleomycin-Treated Chimeric Thy1-Deficient Mice with Thy1-Deficient Myofibroblasts and Thy-Positive Lymphocytes Resolve Inflammation without Affecting the Fibrotic Response. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1072649
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072649