Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes
Joint Authors
Prato, Mauro
Giribaldi, Giuliana
Gulino, Giulia Rossana
Magnetto, Chiara
Khadjavi, Amina
Panariti, Alice
Soster, Marco
Argenziano, Monica
Guiot, Caterina
Cavalli, Roberta
Rivolta, Ilaria
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-23
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Monocytes play a key role in the inflammatory stage of the healing process.
To allow monocyte migration to injured tissues, the balances between secreted matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) must be finely modulated.
However, a reduction of blood supply and local oxygen tension can modify the phenotype of immune cells.
Intriguingly, hypoxia might be targeted by new effective oxygenating devices such as 2H,3H-decafluoropentane- (DFP-) based oxygen-loaded nanodroplets (OLNs).
Here, hypoxia effects on gelatinase/TIMP release from human peripheral monocytes were investigated, and the therapeutic potential of dextran-shelled OLNs was evaluated.
Normoxic monocytes constitutively released ~500 ng/mL MMP-9, ~1.3 ng/mL TIMP-1, and ~0.6 ng/mL TIMP-2 proteins.
MMP-2 was not detected.
After 24 hours, hypoxia significantly altered MMP-9/TIMP-1 balance by reducing MMP-9 and increasing TIMP-1, without affecting TIMP-2 secretion.
Interestingly OLNs, not displaying toxicity to human monocytes after cell internalization, effectively counteracted hypoxia, restoring a normoxia-like MMP-9/TIMP-1 ratio.
The action of OLNs was specifically dependent on time-sustained oxygen diffusion up to 24 h from their DFP-based core.
Therefore, OLNs appear as innovative, nonconventional, cost-effective, and nontoxic therapeutic tools, to be potentially employed to restore the physiological invasive phenotype of immune cells in hypoxia-associated inflammation.
American Psychological Association (APA)
Gulino, Giulia Rossana& Magnetto, Chiara& Khadjavi, Amina& Panariti, Alice& Rivolta, Ilaria& Soster, Marco…[et al.]. 2015. Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072666
Modern Language Association (MLA)
Gulino, Giulia Rossana…[et al.]. Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes. Mediators of Inflammation No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1072666
American Medical Association (AMA)
Gulino, Giulia Rossana& Magnetto, Chiara& Khadjavi, Amina& Panariti, Alice& Rivolta, Ilaria& Soster, Marco…[et al.]. Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072666
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072666