Blockage of Eosinopoiesis by IL-17A Is Prevented by Cytokine and Lipid Mediators of Allergic Inflammation
Joint Authors
Cunha, Fernando Queiroz
Xavier-Elsas, P.
Vieira, B. M.
Masid-de-Brito, D.
Gaspar-Elsas, M. I.
Alves-Filho, José C.
Queto, Túlio
de Luca, Bianca
Dahab, Elizabeth Chen
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-23
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Interleukin- (IL-) 17A, a pleiotropic mediator of inflammation and autoimmunity, potently stimulates bone-marrow neutrophil production.
To explore IL-17A effects on eosinopoiesis, we cultured bone-marrow from wild-type mice, or mutants lacking inducible nitric oxide synthase (iNOS−/−), CD95 (lpr), IL-17RA, or IL-4, with IL-5, alone or associated with IL-17A.
Synergisms between IL-17A-activated, NO-dependent, and NO-independent mechanisms and antagonisms between IL-17A and proallergic factors were further examined.
While IL-17A (0.1–10 ng/mL) had no IL-5-independent effect on eosinopoiesis, it dose-dependently suppressed IL-5-induced eosinophil differentiation, by acting during the initial 24 hours.
Its effectiveness was abolished by caspase inhibitor, zVAD-fmk.
The effect of IL-17A (0.1–1 ng/mL) was sensitive to the iNOS-selective inhibitor aminoguanidine and undetectable in iNOS−/− bone-marrow.
By contrast, a higher IL-17A concentration (10 ng/mL) retained significant suppressive effect in both conditions, unmasking a high-end iNOS-independent mechanism.
Lower IL-17A concentrations synergized with NO donor nitroprusside.
Eosinopoiesis suppression by IL-17A was (a) undetectable in bone-marrow lacking IL-17RA or CD95 and (b) actively prevented by LTD4, LTC4, IL-13, and eotaxin.
Sensitivity to IL-17A was increased in bone-marrow lacking IL-4; adding IL-4 to the cultures restored IL-5 responses to control levels.
Therefore, effects of both IL-17A and proallergic factors are transduced by the iNOS-CD95 pathway in isolated bone-marrow.
American Psychological Association (APA)
Xavier-Elsas, P.& de Luca, Bianca& Queto, Túlio& Vieira, B. M.& Masid-de-Brito, D.& Dahab, Elizabeth Chen…[et al.]. 2015. Blockage of Eosinopoiesis by IL-17A Is Prevented by Cytokine and Lipid Mediators of Allergic Inflammation. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072669
Modern Language Association (MLA)
Xavier-Elsas, P.…[et al.]. Blockage of Eosinopoiesis by IL-17A Is Prevented by Cytokine and Lipid Mediators of Allergic Inflammation. Mediators of Inflammation No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1072669
American Medical Association (AMA)
Xavier-Elsas, P.& de Luca, Bianca& Queto, Túlio& Vieira, B. M.& Masid-de-Brito, D.& Dahab, Elizabeth Chen…[et al.]. Blockage of Eosinopoiesis by IL-17A Is Prevented by Cytokine and Lipid Mediators of Allergic Inflammation. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072669
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072669