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Long-Term Spatiotemporal Reconfiguration of Neuronal Activity Revealed by Voltage-Sensitive Dye Imaging in the Cerebellar Granular Layer
Joint Authors
D'Angelo, Egidio
Gandolfi, Daniela
Mapelli, Jonathan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Understanding the spatiotemporal organization of long-term synaptic plasticity in neuronal networks demands techniques capable of monitoring changes in synaptic responsiveness over extended multineuronal structures.
Among these techniques, voltage-sensitive dye imaging (VSD imaging) is of particular interest due to its good spatial resolution.
However, improvements of the technique are needed in order to overcome limits imposed by its low signal-to-noise ratio.
Here, we show that VSD imaging can detect long-term potentiation (LTP) and long-term depression (LTD) in acute cerebellar slices.
Combined VSD imaging and patch-clamp recordings revealed that the most excited regions were predominantly associated with granule cells (GrCs) generating EPSP-spike complexes, while poorly responding regions were associated with GrCs generating EPSPs only.
The correspondence with cellular changes occurring during LTP and LTD was highlighted by a vector representation obtained by combining amplitude with time-to-peak of VSD signals.
This showed that LTP occurred in the most excited regions lying in the core of activated areas and increased the number of EPSP-spike complexes, while LTD occurred in the less excited regions lying in the surround.
VSD imaging appears to be an efficient tool for investigating how synaptic plasticity contributes to the reorganization of multineuronal activity in neuronal circuits.
American Psychological Association (APA)
Gandolfi, Daniela& Mapelli, Jonathan& D'Angelo, Egidio. 2015. Long-Term Spatiotemporal Reconfiguration of Neuronal Activity Revealed by Voltage-Sensitive Dye Imaging in the Cerebellar Granular Layer. Neural Plasticity،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1075314
Modern Language Association (MLA)
Gandolfi, Daniela…[et al.]. Long-Term Spatiotemporal Reconfiguration of Neuronal Activity Revealed by Voltage-Sensitive Dye Imaging in the Cerebellar Granular Layer. Neural Plasticity No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1075314
American Medical Association (AMA)
Gandolfi, Daniela& Mapelli, Jonathan& D'Angelo, Egidio. Long-Term Spatiotemporal Reconfiguration of Neuronal Activity Revealed by Voltage-Sensitive Dye Imaging in the Cerebellar Granular Layer. Neural Plasticity. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1075314
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075314