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Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis
Joint Authors
Zhang, Jifeng
Tan, Minghui
Yin, Yichen
Ren, Bingyu
Jiang, Nannan
Guo, Guoqing
Chen, Yuan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Endophilin isoforms perform distinct characteristics in their interactions with N-type Ca2+ channels and dynamin.
However, precise functional differences for the endophilin isoforms on synaptic vesicle (SV) endocytosis remain unknown.
By coupling RNA interference and electrophysiological recording techniques in cultured rat hippocampal neurons, we investigated the functional differences of three isoforms of endophilin in SV endocytosis.
The results showed that the amplitude of normalized evoked excitatory postsynaptic currents in endophilin1 knockdown neurons decreased significantly for both single train and multiple train stimulations.
Similar results were found using endophilin2 knockdown neurons, whereas endophilin3 siRNA exhibited no change compared with control neurons.
Endophilin1 and endophilin2 affected SV endocytosis, but the effect of endophilin1 and endophilin2 double knockdown was not different from that of either knockdown alone.
This result suggested that endophilin1 and endophilin2 functioned together but not independently during SV endocytosis.
Taken together, our results indicate that SV endocytosis is sustained by endophilin1 and endophilin2 isoforms, but not by endophilin3, in primary cultured hippocampal neurons.
American Psychological Association (APA)
Zhang, Jifeng& Tan, Minghui& Yin, Yichen& Ren, Bingyu& Jiang, Nannan& Guo, Guoqing…[et al.]. 2015. Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis. Neural Plasticity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075329
Modern Language Association (MLA)
Zhang, Jifeng…[et al.]. Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis. Neural Plasticity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075329
American Medical Association (AMA)
Zhang, Jifeng& Tan, Minghui& Yin, Yichen& Ren, Bingyu& Jiang, Nannan& Guo, Guoqing…[et al.]. Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis. Neural Plasticity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075329
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075329