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Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B
Joint Authors
Batassini, Cristiane
Tortorelli, Lucas Silva
Broetto, Núbia
Borsoi, Milene
Zanotto, Caroline
Galland, Fabiana
Souza, Tadeu Mello
Leite, Marina Concli
Gonçalves, Carlos Alberto
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Both glial fibrillary acidic protein (GFAP) and S100B have been used as markers of astroglial plasticity, particularly in brain injury; however, they do not necessarily change in the same time frame or direction.
Herein, we induced a Parkinson’s disease (PD) model via a 6-OHDA intrastriatal injection in rats and investigated the changes in GFAP and S100B using ELISA in the substantia nigra (SN), striatum, and cerebrospinal fluid on the 1st, 7th, and 21st days following the injection.
The model was validated using measurements of rotational behaviour induced by methylphenidate and tyrosine hydroxylase in the dopaminergic pathway.
To our knowledge, this is the first measurement of cerebrospinal fluid S100B and GFAP in the 6-OHDA model of PD.
Gliosis (based on a GFAP increase) was identified in the striatum, but not in the SN.
We identified a transitory increment of cerebrospinal fluid S100B and GFAP on the 1st and 7th days, respectively.
This initial change in cerebrospinal fluid S100B was apparently related to the mechanical lesion.
However, the 6-OHDA-induced S100B secretion was confirmed in astrocyte cultures.
Current data reinforce the idea that glial changes precede neuronal damage in PD; however, these findings also indicate that caution is necessary regarding the interpretation of data in this PD model.
American Psychological Association (APA)
Batassini, Cristiane& Broetto, Núbia& Tortorelli, Lucas Silva& Borsoi, Milene& Zanotto, Caroline& Galland, Fabiana…[et al.]. 2015. Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B. Neural Plasticity،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075333
Modern Language Association (MLA)
Batassini, Cristiane…[et al.]. Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B. Neural Plasticity No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1075333
American Medical Association (AMA)
Batassini, Cristiane& Broetto, Núbia& Tortorelli, Lucas Silva& Borsoi, Milene& Zanotto, Caroline& Galland, Fabiana…[et al.]. Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B. Neural Plasticity. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075333
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075333