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CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation
Joint Authors
Zhang, Jifeng
Tan, Minghui
Guo, Guoqing
Cha, Caihui
Ye, Yongheng
Li, Sumei
Wu, Fengming
Gong, Sitang
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Cytoskeleton dynamics are critical phenomena that underpin many fundamental cellular processes.
Collapsin response mediator proteins (CRMPs) are highly expressed in the developing nervous system, mediating growth cone guidance, neuronal polarity, and axonal elongation.
However, whether and how CRMPs associate with microtubules and actin coordinated cytoskeletal dynamics remain unknown.
In this study, we demonstrated that CRMP2 and CRMP4 interacted with tubulin and actin in vitro and colocalized with the cytoskeleton in the transition-zone in developing growth cones.
CRMP2 and CRMP4 also interacted with one another coordinately to promote growth cone development and axonal elongation.
Genetic silencing of CRMP2 enhanced, whereas overexpression of CRMP2 suppressed, the inhibitory effects of CRMP4 knockdown on axonal development.
In addition, knockdown of CRMP2 or overexpression of truncated CRMP2 reversed the promoting effect of CRMP4.
With the overexpression of truncated CRMP2 or CRMP4 lacking the cytoskeleton interaction domain, the promoting effect of CRMP was suppressed.
These data suggest a model in which CRMP2 and CRMP4 form complexes to bridge microtubules and actin and thus work cooperatively to regulate growth cone development and axonal elongation.
American Psychological Association (APA)
Tan, Minghui& Cha, Caihui& Ye, Yongheng& Zhang, Jifeng& Li, Sumei& Wu, Fengming…[et al.]. 2015. CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation. Neural Plasticity،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1075431
Modern Language Association (MLA)
Tan, Minghui…[et al.]. CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation. Neural Plasticity No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1075431
American Medical Association (AMA)
Tan, Minghui& Cha, Caihui& Ye, Yongheng& Zhang, Jifeng& Li, Sumei& Wu, Fengming…[et al.]. CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation. Neural Plasticity. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1075431
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075431