Propofol Attenuates Small Intestinal Ischemia Reperfusion Injury through Inhibiting NADPH Oxidase Mediated Mast Cell Activation
Joint Authors
Irwin, Michael G.
Li, Haobo
Luo, Chen-fang
Xing, Dandan
Su, Guangjie
Li, Shun
Gan, Xiaoliang
Xia, Zhengyuan
Hei, Ziqing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-12
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Both oxidative stress and mast cell (MC) degranulation participate in the process of small intestinal ischemia reperfusion (IIR) injury, and oxidative stress induces MC degranulation.
Propofol, an anesthetic with antioxidant property, can attenuate IIR injury.
We postulated that propofol can protect against IIR injury by inhibiting oxidative stress subsequent from NADPH oxidase mediated MC activation.
Cultured RBL-2H3 cells were pretreated with antioxidant N-acetylcysteine (NAC) or propofol and subjected to hydrogen peroxide (H2O2) stimulation without or with MC degranulator compound 48/80 (CP).
H2O2 significantly increased cells degranulation, which was abolished by NAC or propofol.
MC degranulation by CP further aggravated H2O2 induced cell degranulation of small intestinal epithelial cell, IEC-6 cells, stimulated by tryptase.
Rats subjected to IIR showed significant increases in cellular injury and elevations of NADPH oxidase subunits p 4 7 phox and gp 9 1 phox protein expression, increases of the specific lipid peroxidation product 15- F 2 t -Isoprostane and interleukin-6, and reductions in superoxide dismutase activity with concomitant enhancements in tryptase and β-hexosaminidase.
MC degranulation by CP further aggravated IIR injury.
And all these changes were attenuated by NAC or propofol pretreatment, which also abrogated CP-mediated exacerbation of IIR injury.
It is concluded that pretreatment of propofol confers protection against IIR injury by suppressing NADPH oxidase mediated MC activation.
American Psychological Association (APA)
Gan, Xiaoliang& Xing, Dandan& Su, Guangjie& Li, Shun& Luo, Chen-fang& Irwin, Michael G.…[et al.]. 2015. Propofol Attenuates Small Intestinal Ischemia Reperfusion Injury through Inhibiting NADPH Oxidase Mediated Mast Cell Activation. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1075541
Modern Language Association (MLA)
Gan, Xiaoliang…[et al.]. Propofol Attenuates Small Intestinal Ischemia Reperfusion Injury through Inhibiting NADPH Oxidase Mediated Mast Cell Activation. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1075541
American Medical Association (AMA)
Gan, Xiaoliang& Xing, Dandan& Su, Guangjie& Li, Shun& Luo, Chen-fang& Irwin, Michael G.…[et al.]. Propofol Attenuates Small Intestinal Ischemia Reperfusion Injury through Inhibiting NADPH Oxidase Mediated Mast Cell Activation. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1075541
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075541