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Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation
Joint Authors
Capó, Xavier
Martorell, Miquel
Sureda, Antoni
Tur, Josep Antoni
Pons, Antoni
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
We studied the effects of diet supplementation with docosahexaenoic (DHA) and in vitro vitamin C (VitC) at physiological concentrations on oxidative and inflammatory neutrophil response to phorbol myristate acetate (PMA).
Fifteen male footballers ingested a beverage enriched with DHA or a placebo for 8 weeks in a randomized double-blind study.
Neutrophils were isolated from blood samples collected in basal conditions at the end of nutritional intervention.
Neutrophils were cultured for 2 hours at 37°C in (a) control media, (b) media with PMA, and (c) media with PMA + VitC.
PMA induces neutrophil degranulation with increased extracellular myeloperoxidase and catalase activities, nitric oxide production, expression of the inflammatory genes cyclooxygenase-2, nuclear factor κβ, interleukin 8 and tumor necrosis factor α, and interleukin 6 production.
DHA diet supplementation boosts the exit of CAT from neutrophils but moderates the degranulation of myeloperoxidase granules induced by PMA.
VitC facilitates azurophilic degranulation of neutrophils and increases gene expression of myeloperoxidase induced by PMA.
VitC and DHA diet supplementation prevent PMA effects on inflammatory gene expression, although together they do not produce additional effects.
DHA diet supplementation enhances antioxidant defences and anti-inflammatory neutrophil response to in vitro PMA activation.
VitC facilitates neutrophil degranulation but prevents an inflammatory response to PMA.
American Psychological Association (APA)
Capó, Xavier& Martorell, Miquel& Sureda, Antoni& Tur, Josep Antoni& Pons, Antoni. 2015. Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1075553
Modern Language Association (MLA)
Capó, Xavier…[et al.]. Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1075553
American Medical Association (AMA)
Capó, Xavier& Martorell, Miquel& Sureda, Antoni& Tur, Josep Antoni& Pons, Antoni. Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1075553
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075553