Smooth Muscle Specific Overexpression of p22phox Potentiates Carotid Artery Wall Thickening in Response to Injury
Joint Authors
Manogue, Michael R.
Bennett, Justin R.
Holland, Drury S.
Choi, Chung-Sik
Drake, Douglas A.
Taylor, Mark S.
Weber, David S.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We hypothesized that transgenic mice overexpressing the p22phox subunit of the NADPH oxidase selectively in smooth muscle (Tgp22smc) would exhibit an exacerbated response to transluminal carotid injury compared to wild-type mice.
To examine the role of reactive oxygen species (ROS) as a mediator of vascular injury, the injury response was quantified by measuring wall thickness (WT) and cross-sectional wall area (CSWA) of the injured and noninjured arteries in both Tgp22smc and wild-type animals at days 3, 7, and 14 after injury.
Akt, p38 MAPK, and Src activation were evaluated at the same time points using Western blotting.
WT and CSWA following injury were significantly greater in Tgp22smc mice at both 7 and 14 days after injury while noninjured contralateral carotids were similar between groups.
Apocynin treatment attenuated the injury response in both groups and rendered the response similar between Tgp22smc mice and wild-type mice.
Following injury, carotid arteries from Tgp22smc mice demonstrated elevated activation of Akt at day 3, while p38 MAPK and Src activation was elevated at day 7 compared to wild-type mice.
Both increased activation and temporal regulation of these signaling pathways may contribute to enhanced vascular growth in response to injury in this transgenic model of elevated vascular ROS.
American Psychological Association (APA)
Manogue, Michael R.& Bennett, Justin R.& Holland, Drury S.& Choi, Chung-Sik& Drake, Douglas A.& Taylor, Mark S.…[et al.]. 2015. Smooth Muscle Specific Overexpression of p22phox Potentiates Carotid Artery Wall Thickening in Response to Injury. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075583
Modern Language Association (MLA)
Manogue, Michael R.…[et al.]. Smooth Muscle Specific Overexpression of p22phox Potentiates Carotid Artery Wall Thickening in Response to Injury. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075583
American Medical Association (AMA)
Manogue, Michael R.& Bennett, Justin R.& Holland, Drury S.& Choi, Chung-Sik& Drake, Douglas A.& Taylor, Mark S.…[et al.]. Smooth Muscle Specific Overexpression of p22phox Potentiates Carotid Artery Wall Thickening in Response to Injury. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075583
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075583