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L-Lactate Protects Skin Fibroblasts against Aging-Associated Mitochondrial Dysfunction via Mitohormesis
Joint Authors
Zelenka, Jaroslav
Dvořák, Aleš
Alán, Lukáš
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-10
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
A moderate elevation of reactive oxygen species (ROS) production and a mild inhibition of mitochondrial respiratory chain have been associated with a health promotion and a lifespan extension in several animal models of aging.
Here, we tested whether this phenomenon called mitohormesis could be mediated by L-lactate.
The treatment with 5 mM L-lactate significantly increased H2O2 production and slightly inhibited the respiration in cultured skin fibroblasts and in isolated mitochondria.
The L-lactate exposure was associated with oxidation of intracellular glutathione, phosphorylation of 5′AMP-activated protein kinase (AMPK), and induction of peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1α) transcription.
A replicative aging of fibroblasts (L0) with a constant (LC), or intermittent 5 mM L-lactate (LI) in media showed that the high-passage LI fibroblasts have higher respiration, lower H2O2 release, and lower secretion of L-lactate compared to L0 and LC.
This protection against mitochondrial dysfunction in LI cells was associated with lower activity of mechanistic target of rapamycin complex 1 (mTORC1), less signs of cellular senescence, and increased autophagy compared to L0 and LC.
In conclusion, we demonstrated that intermittent but not constant exposure to L-lactate triggers mitohormesis, prevents aging-associated mitochondrial dysfunction, and improves other markers of aging.
American Psychological Association (APA)
Zelenka, Jaroslav& Dvořák, Aleš& Alán, Lukáš. 2015. L-Lactate Protects Skin Fibroblasts against Aging-Associated Mitochondrial Dysfunction via Mitohormesis. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1075596
Modern Language Association (MLA)
Zelenka, Jaroslav…[et al.]. L-Lactate Protects Skin Fibroblasts against Aging-Associated Mitochondrial Dysfunction via Mitohormesis. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1075596
American Medical Association (AMA)
Zelenka, Jaroslav& Dvořák, Aleš& Alán, Lukáš. L-Lactate Protects Skin Fibroblasts against Aging-Associated Mitochondrial Dysfunction via Mitohormesis. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1075596
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075596