Toll-Like Receptor 4 Promotes NO Synthesis by Upregulating GCHI Expression under Oxidative Stress Conditions in Sheep MonocytesMacrophages
Joint Authors
Deng, Shoulong
Yu, Kun
Zhang, Baolu
Yao, Yuchang
Wang, Zhixian
Zhang, Jinlong
Zhang, Xiaosheng
Liu, Guoshi
Li, Ning
Liu, Yixun
Lian, Zhengxing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Many groups of Gram-negative bacteria cause diseases that are harmful to sheep.
Toll-like receptor 4 (TLR4), which is critical for detecting Gram-negative bacteria by the innate immune system, is activated by lipopolysaccharide (LPS) to initiate inflammatory responses and oxidative stress.
Oxidation intermediates are essential activators of oxidative stress, as low levels of free radicals form a stressful oxidative environment that can clear invading pathogens.
NO is an oxidation intermediate and its generation is regulated by nitric oxide synthase (iNOS).
Guanosine triphosphate cyclohydrolase (GCHI) is the rate-limiting enzyme for tetrahydrobiopterin (BH4) synthesis, which is essential for the production of inducible iNOS.
Previously, we made vectors to overexpress the sheep TLR4 gene.
Herein, first generation (G1) of transgenic sheep was stimulated with LPS in vivo and in vitro, and oxidative stress and GCHI expression were investigated.
Oxidative injury caused by TLR4 overexpression was tightly regulated in tissues.
However, the transgenic (Tg) group still secreted nitric oxide (NO) when an iNOS inhibitor was added.
Furthermore, GCHI expression remained upregulated in both serum and monocytes/macrophages.
Thus, overexpression of TLR4 in transgenic sheep might accelerate the clearance of invading microbes through NO generation following LPS stimulation.
Additionally, TLR4 overexpression also enhances GCHI activation.
American Psychological Association (APA)
Deng, Shoulong& Yu, Kun& Zhang, Baolu& Yao, Yuchang& Wang, Zhixian& Zhang, Jinlong…[et al.]. 2015. Toll-Like Receptor 4 Promotes NO Synthesis by Upregulating GCHI Expression under Oxidative Stress Conditions in Sheep MonocytesMacrophages. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075601
Modern Language Association (MLA)
Deng, Shoulong…[et al.]. Toll-Like Receptor 4 Promotes NO Synthesis by Upregulating GCHI Expression under Oxidative Stress Conditions in Sheep MonocytesMacrophages. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1075601
American Medical Association (AMA)
Deng, Shoulong& Yu, Kun& Zhang, Baolu& Yao, Yuchang& Wang, Zhixian& Zhang, Jinlong…[et al.]. Toll-Like Receptor 4 Promotes NO Synthesis by Upregulating GCHI Expression under Oxidative Stress Conditions in Sheep MonocytesMacrophages. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075601
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075601