Glucose Oxidase Induces Cellular Senescence in Immortal Renal Cells through ILK by Downregulating Klotho Gene Expression
Joint Authors
Troyano-Suárez, Nuria
del Nogal-Avila, María
Mora, Inés
Sosa, Patricia
López-Ongil, Susana
Rodriguez-Puyol, Diego
Olmos, Gemma
Ruíz-Torres, María Piedad
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-25
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Cellular senescence can be prematurely induced by oxidative stress involved in aging.
In this work, we were searching for novel intermediaries in oxidative stress-induced senescence, focusing our interest on integrin-linked kinase (ILK), a scaffold protein at cell-extracellular matrix (ECM) adhesion sites, and on the Klotho gene.
Cultured renal cells were treated with glucose oxidase (GOx) for long time periods.
GOx induced senescence, increasing senescence associated β-galactosidase activity and the expression of p16.
In parallel, GOx increased ILK protein expression and activity.
Ectopic overexpression of ILK in cells increased p16 expression, even in the absence of GOx, whereas downregulation of ILK inhibited the increase in p16 due to oxidative stress.
Additionally, GOx reduced Klotho gene expression and cells overexpressing Klotho protein did not undergo senescence after GOx addition.
We demonstrated a direct link between ILK and Klotho since silencing ILK expression in cells and mice increases Klotho expression and reduces p53 and p16 expression in renal cortex.
In conclusion, oxidative stress induces cellular senescence in kidney cells by increasing ILK protein expression and activity, which in turn reduces Klotho expression.
We hereby present ILK as a novel downregulator of Klotho gene expression.
American Psychological Association (APA)
Troyano-Suárez, Nuria& del Nogal-Avila, María& Mora, Inés& Sosa, Patricia& López-Ongil, Susana& Rodriguez-Puyol, Diego…[et al.]. 2015. Glucose Oxidase Induces Cellular Senescence in Immortal Renal Cells through ILK by Downregulating Klotho Gene Expression. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1075645
Modern Language Association (MLA)
Troyano-Suárez, Nuria…[et al.]. Glucose Oxidase Induces Cellular Senescence in Immortal Renal Cells through ILK by Downregulating Klotho Gene Expression. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1075645
American Medical Association (AMA)
Troyano-Suárez, Nuria& del Nogal-Avila, María& Mora, Inés& Sosa, Patricia& López-Ongil, Susana& Rodriguez-Puyol, Diego…[et al.]. Glucose Oxidase Induces Cellular Senescence in Immortal Renal Cells through ILK by Downregulating Klotho Gene Expression. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1075645
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075645