TrkB-Mediated Neuroprotective and Antihypoxic Properties of Brain-Derived Neurotrophic Factor
Joint Authors
Vedunova, M.
Mishchenko, Tatiana A.
Mitroshina, Elena V.
Mukhina, Irina V.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The neuroprotective and antihypoxic effects of brain-derived neurotrophic factor (BDNF) on dissociated hippocampal cultures in a hypoxia model were investigated.
These experiments demonstrate that 10 minutes of normobaric hypoxia increased the number of dead cells in primary culture, whereas a preventive application of BDNF increased the number of viable cells.
Spontaneous bioelectrical and calcium activity in neural networks was analyzed using multielectrode arrays and functional intravital calcium imaging.
The results indicate that BDNF affects the functional parameters of neuronal networks in dissociated hippocampal cultures over the 7-day posthypoxic period.
In addition, the effects of k252a, an antagonist of tropomyosin-related kinase B (TrkB), on functional bioelectrical activity during and after acute hypoxia were investigated.
It was shown that the protective effects of BDNF are associated with binding to the TrkB receptor.
Finally, intravital fluorescent mRNA probes were used to study the role of NF-κB1 in the protective effects of BDNF.
Our experiments revealed that BDNF application stimulates NF-κB1 mRNA synthesis in primary dissociated hippocampal cells under normal conditions but not in hypoxic state.
American Psychological Association (APA)
Vedunova, M.& Mishchenko, Tatiana A.& Mitroshina, Elena V.& Mukhina, Irina V.. 2015. TrkB-Mediated Neuroprotective and Antihypoxic Properties of Brain-Derived Neurotrophic Factor. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075657
Modern Language Association (MLA)
Vedunova, M.…[et al.]. TrkB-Mediated Neuroprotective and Antihypoxic Properties of Brain-Derived Neurotrophic Factor. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1075657
American Medical Association (AMA)
Vedunova, M.& Mishchenko, Tatiana A.& Mitroshina, Elena V.& Mukhina, Irina V.. TrkB-Mediated Neuroprotective and Antihypoxic Properties of Brain-Derived Neurotrophic Factor. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075657
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075657