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In Vitro Toxicity of Epigallocatechin Gallate in Rat Liver Mitochondria and Hepatocytes
Joint Authors
Kučera, Otto
Endlicher, René
Lotková, Halka
Drahota, Zdeněk
Červinková, Zuzana
Mezera, Vojtech
Moravcova, Alena
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Epigallocatechin-3-gallate (EGCG) is the main compound of green tea with well-described antioxidant, anti-inflammatory, and tumor-suppressing properties.
However, EGCG at high doses was reported to cause liver injury.
In this study, we evaluated the effect of EGCG on primary culture of rat hepatocytes and on rat liver mitochondria in permeabilized hepatocytes.
The 24-hour incubation with EGCG in concentrations of 10 μmol/L and higher led to signs of cellular injury and to a decrease in hepatocyte functions.
The effect of EGCG on the formation of reactive oxygen species (ROS) was biphasic.
While low doses of EGCG decreased ROS production, the highest tested dose induced a significant increase in ROS formation.
Furthermore, we observed a decline in mitochondrial membrane potential in cells exposed to EGCG when compared to control cells.
In permeabilized hepatocytes, EGCG caused damage of the outer mitochondrial membrane and an uncoupling of oxidative phosphorylation.
EGCG in concentrations lower than 10 μmol/L was recognized as safe for hepatocytes in vitro.
American Psychological Association (APA)
Kučera, Otto& Mezera, Vojtech& Moravcova, Alena& Endlicher, René& Lotková, Halka& Drahota, Zdeněk…[et al.]. 2015. In Vitro Toxicity of Epigallocatechin Gallate in Rat Liver Mitochondria and Hepatocytes. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075663
Modern Language Association (MLA)
Kučera, Otto…[et al.]. In Vitro Toxicity of Epigallocatechin Gallate in Rat Liver Mitochondria and Hepatocytes. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075663
American Medical Association (AMA)
Kučera, Otto& Mezera, Vojtech& Moravcova, Alena& Endlicher, René& Lotková, Halka& Drahota, Zdeněk…[et al.]. In Vitro Toxicity of Epigallocatechin Gallate in Rat Liver Mitochondria and Hepatocytes. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075663
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075663