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Doses of Quercetin in the Range of Serum Concentrations Exert Delipidating Effects in 3T3-L1 Preadipocytes by Acting on Different Stages of Adipogenesis, but Not in Mature Adipocytes
Joint Authors
Eseberri, Itziar
Miranda, Jonatan
Lasa, Arrate
Churruca, Itziar
Portillo, María P.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Scope.
To determine whether doses of quercetin in the range of serum concentrations exert any effect on triacylglycerol accumulation in maturing preadipocytes and mature adipocytes.
The influence on the expression of adipogenic markers as well as on gene expression and activity of enzymes involved in triacylglycerol metabolism were assessed.
Methods and Results.
3T3-L1 preadipocytes were treated during differentiation and mature adipocytes for 24 hours with low doses (0.1–10 µM) of quercetin.
Triacylglycerol content in both cell types and free fatty acid and glycerol in the incubation medium of mature adipocytes were measured spectrophotometrically.
Gene and protein expression were assessed by RT-PCR and Western blot.
LPL and FAS activities were quantified.
During differentiation quercetin reduced triacylglycerol content at doses from 0.5 to 10 µM.
1 µM of quercetin reduced C/EBPβ gene expression, SREBP1 mature protein levels, and PPARγ gene expression.
10 µM of quercetin reduced LPL gene expression and PPARγ and SREBP1c expression.
In mature adipocytes, only 10 µM of quercetin reduced triacylglycerol content.
Lipogenic FAS expression and activity were reduced at this dose.
Conclusion.
Quercetin, in the range of serum concentrations, is able to inhibit adipogenesis, but higher doses, at least 10 µM, are needed to reduce fat accumulation in mature adipocytes.
American Psychological Association (APA)
Eseberri, Itziar& Miranda, Jonatan& Lasa, Arrate& Churruca, Itziar& Portillo, María P.. 2015. Doses of Quercetin in the Range of Serum Concentrations Exert Delipidating Effects in 3T3-L1 Preadipocytes by Acting on Different Stages of Adipogenesis, but Not in Mature Adipocytes. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075665
Modern Language Association (MLA)
Eseberri, Itziar…[et al.]. Doses of Quercetin in the Range of Serum Concentrations Exert Delipidating Effects in 3T3-L1 Preadipocytes by Acting on Different Stages of Adipogenesis, but Not in Mature Adipocytes. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1075665
American Medical Association (AMA)
Eseberri, Itziar& Miranda, Jonatan& Lasa, Arrate& Churruca, Itziar& Portillo, María P.. Doses of Quercetin in the Range of Serum Concentrations Exert Delipidating Effects in 3T3-L1 Preadipocytes by Acting on Different Stages of Adipogenesis, but Not in Mature Adipocytes. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075665
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075665