Protective Effects of Kaempferol against Myocardial IschemiaReperfusion Injury in Isolated Rat Heart via Antioxidant Activity and Inhibition of Glycogen Synthase Kinase-3β
Joint Authors
Zheng, Qiusheng
Wang, Dong
Zhou, Mingjie
Han, Jichun
Ren, Huanhuan
Wang, Wenjuan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
This study aimed to evaluate the protective effect of kaempferol against myocardial ischemia/reperfusion (I/R) injury in rats.
Method.
Left ventricular developed pressure (LVDP) and its maximum up/down rate (±dp/dtmax) were recorded as myocardial function.
Infarct size was detected with 2,3,5-triphenyltetrazolium chloride staining.
Cardiomyocyte apoptosis was determined using terminal deoxynucleotidyl nick-end labeling (TUNEL).
The levels of creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione/glutathione disulfide (GSH/GSSG) ratio, and tumor necrosis factor-alpha (TNF-α) were determined using enzyme linked immunosorbent assay (ELISA).
Moreover, total glycogen synthase kinase-3β (GSK-3β), phospho-GSK-3β (P-GSK-3β), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis.
Results.
Pretreatment with kaempferol significantly improved the recovery of LVDP and ±dp/dtmax, as well as increased the levels of SOD and P-GSK-3β and GSH/GSSG ratio.
However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-α.
Conclusion.
These results suggested that kaempferol provides cardioprotection via antioxidant activity and inhibition of GSK-3β activity in rats with I/R.
American Psychological Association (APA)
Zhou, Mingjie& Ren, Huanhuan& Han, Jichun& Wang, Wenjuan& Zheng, Qiusheng& Wang, Dong. 2015. Protective Effects of Kaempferol against Myocardial IschemiaReperfusion Injury in Isolated Rat Heart via Antioxidant Activity and Inhibition of Glycogen Synthase Kinase-3β. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075666
Modern Language Association (MLA)
Zhou, Mingjie…[et al.]. Protective Effects of Kaempferol against Myocardial IschemiaReperfusion Injury in Isolated Rat Heart via Antioxidant Activity and Inhibition of Glycogen Synthase Kinase-3β. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1075666
American Medical Association (AMA)
Zhou, Mingjie& Ren, Huanhuan& Han, Jichun& Wang, Wenjuan& Zheng, Qiusheng& Wang, Dong. Protective Effects of Kaempferol against Myocardial IschemiaReperfusion Injury in Isolated Rat Heart via Antioxidant Activity and Inhibition of Glycogen Synthase Kinase-3β. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075666
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075666