DNA Damage and Inhibition of Akt Pathway in MCF-7 Cells and Ehrlich Tumor in Mice Treated with 1,4-Naphthoquinones in Combination with Ascorbate
Joint Authors
Ourique, Fabiana
Kviecinski, Maicon R.
Felipe, Karina B.
Correia, João Francisco Gomes
Farias, Mirelle S.
Castro, Luiza S. E. P. W.
Grinevicius, Valdelúcia M. A. S.
Valderrama, Jaime
Rios, David
Benites, Julio
Buc Calderon, Pedro
Pedrosa, Rozangela Curi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of this study was to enhance the understanding of the antitumor mechanism of 1,4-naphthoquinones and ascorbate.
Juglone, phenylaminonaphthoquinone-7, and 9 (Q7/Q9) were evaluated for effects on CT-DNA and DNA of cancer cells.
Evaluations in MCF-7 cells are DNA damage, ROS levels, viability, and proliferation.
Proteins from MCF-7 lysates were immunoblotted for verifying PARP integrity, γH2AX, and pAkt.
Antitumor activity was measured in Ehrlich ascites carcinoma-bearing mice.
The same markers of molecular toxicity were assessed in vivo.
The naphthoquinones intercalate into CT-DNA and caused oxidative cleavage, which is increased in the presence of ascorbate.
Treatments caused DNA damage and reduced viability and proliferation of MCF-7 cells.
Effects were potentiated by ascorbate.
No PARP cleavage was observed.
Naphthoquinones, combined with ascorbate, caused phosphorylation of H2AX and inhibited pAkt.
ROS were enhanced in MCF-7 cells, particularly by the juglone and Q7 plus ascorbate.
Ehrlich carcinoma was inhibited by juglone, Q7, or Q9, but the potentiating effect of ascorbate was reproduced in vivo only in the cases of juglone and Q7, which caused up to 60% inhibition of tumor and the largest extension of survival.
Juglone and Q7 plus ascorbate caused enhanced ROS and DNA damage and inhibited pAkt also in Ehrlich carcinoma cells.
American Psychological Association (APA)
Ourique, Fabiana& Kviecinski, Maicon R.& Felipe, Karina B.& Correia, João Francisco Gomes& Farias, Mirelle S.& Castro, Luiza S. E. P. W.…[et al.]. 2015. DNA Damage and Inhibition of Akt Pathway in MCF-7 Cells and Ehrlich Tumor in Mice Treated with 1,4-Naphthoquinones in Combination with Ascorbate. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075673
Modern Language Association (MLA)
Ourique, Fabiana…[et al.]. DNA Damage and Inhibition of Akt Pathway in MCF-7 Cells and Ehrlich Tumor in Mice Treated with 1,4-Naphthoquinones in Combination with Ascorbate. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075673
American Medical Association (AMA)
Ourique, Fabiana& Kviecinski, Maicon R.& Felipe, Karina B.& Correia, João Francisco Gomes& Farias, Mirelle S.& Castro, Luiza S. E. P. W.…[et al.]. DNA Damage and Inhibition of Akt Pathway in MCF-7 Cells and Ehrlich Tumor in Mice Treated with 1,4-Naphthoquinones in Combination with Ascorbate. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075673
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075673