Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus
Joint Authors
Winyard, Paul G.
Taylor, Emma L.
Armstrong, Kenneth R.
Perrett, David
Hattersley, Andrew T.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Advanced oxidation protein products (AOPP) are reportedly elevated in the plasma of patients with a number of diseases, including diabetes mellitus, that involve oxidative stress.
However, the accurate measurement of AOPP in human plasma is hampered by the formation of a precipitate following the addition of potassium iodide and glacial acetic acid according to the published assay procedure.
Here we describe a modification of the AOPP assay which eliminates interference by precipitation and provides a robust, reliable, and reproducible protocol for the measurement of iodide oxidising capacity in plasma samples (intra-assay CV 1.7–5.3%, interassay CV 5.3–10.5%).
The improved method revealed a significant association of AOPP levels with age (p<0.05) and hypertension (p=0.01) in EDTA-anticoagulated plasma samples from 52 patients with diabetes and 38 nondiabetic control subjects, suggesting a possible link between plasma oxidising capacity and endothelial and/or vascular dysfunction.
There was no significant difference between AOPP concentrations in diabetic (74.8 ± 7.2 μM chloramine T equivalents) and nondiabetic (75.5 ± 7.0 μM chloramine T equivalents) individuals.
American Psychological Association (APA)
Taylor, Emma L.& Armstrong, Kenneth R.& Perrett, David& Hattersley, Andrew T.& Winyard, Paul G.. 2015. Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075674
Modern Language Association (MLA)
Taylor, Emma L.…[et al.]. Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075674
American Medical Association (AMA)
Taylor, Emma L.& Armstrong, Kenneth R.& Perrett, David& Hattersley, Andrew T.& Winyard, Paul G.. Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075674
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075674