Reactive Oxygen Species Are Required for Human Mesenchymal Stem Cells to Initiate Proliferation after the Quiescence Exit
Joint Authors
Borodkina, Aleksandra
Burova, Elena
Shatrova, Alla
Lyublinskaya, O. G.
Borisov, Ya. G.
Pugovkina, N. A.
Smirnova, I. S.
Obidina, Ju. V.
Ivanova, Ju. S.
Zenin, V. V.
Aksenov, N. D.
Zemelko, V. I.
Puzanov, M. V.
Nikolsky, Nikolay
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The present study focuses on the involvement of reactive oxygen species (ROS) in the process of mesenchymal stem cells “waking up” and entering the cell cycle after the quiescence.
Using human endometrial mesenchymal stem cells (eMSCs), we showed that intracellular basal ROS level is positively correlated with the proliferative status of the cell cultures.
Our experiments with the eMSCs synchronized in the G0 phase of the cell cycle revealed a transient increase in the ROS level upon the quiescence exit after stimulation of the cell proliferation.
This increase was registered before the eMSC entry to the S-phase of the cell cycle, and elimination of this increase by antioxidants (N-acetyl-L-cysteine, Tempol, and Resveratrol) blocked G1–S-phase transition.
Similarly, a cell cycle arrest which resulted from the antioxidant treatment was observed in the experiments with synchronized human mesenchymal stem cells derived from the adipose tissue.
Thus, we showed that physiologically relevant level of ROS is required for the initiation of human mesenchymal stem cell proliferation and that low levels of ROS due to the antioxidant treatment can block the stem cell self-renewal.
American Psychological Association (APA)
Lyublinskaya, O. G.& Borisov, Ya. G.& Pugovkina, N. A.& Smirnova, I. S.& Obidina, Ju. V.& Ivanova, Ju. S.…[et al.]. 2015. Reactive Oxygen Species Are Required for Human Mesenchymal Stem Cells to Initiate Proliferation after the Quiescence Exit. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075676
Modern Language Association (MLA)
Lyublinskaya, O. G.…[et al.]. Reactive Oxygen Species Are Required for Human Mesenchymal Stem Cells to Initiate Proliferation after the Quiescence Exit. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1075676
American Medical Association (AMA)
Lyublinskaya, O. G.& Borisov, Ya. G.& Pugovkina, N. A.& Smirnova, I. S.& Obidina, Ju. V.& Ivanova, Ju. S.…[et al.]. Reactive Oxygen Species Are Required for Human Mesenchymal Stem Cells to Initiate Proliferation after the Quiescence Exit. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1075676
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075676