Protective Role of Nuclear Factor E2-Related Factor 2 against Acute Oxidative Stress-Induced Pancreatic β-Cell Damage
Joint Authors
Yang, Bei
Fu, Jingqi
Zheng, Hongzhi
Wang, Huihui
Zhao, Rui
Lu, Chunwei
Liu, Zhiyuan
Hou, Yongyong
Xu, Yuanyuan
Zhang, Qiang
Qu, Weidong
Pi, Jingbo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Oxidative stress is implicated in the pathogenesis of pancreatic β-cell dysfunction that occurs in both type 1 and type 2 diabetes.
Nuclear factor E2-related factor 2 (NRF2) is a master regulator in the cellular adaptive response to oxidative stress.
The present study found that MIN6 β-cells with stable knockdown of Nrf2 (Nrf2-KD) and islets isolated from Nrf2-knockout mice expressed substantially reduced levels of antioxidant enzymes in response to a variety of stressors.
In scramble MIN6 cells or wild-type islets, acute exposure to oxidative stressors, including hydrogen peroxide (H2O2) and S-nitroso-N-acetylpenicillamine, resulted in cell damage as determined by decrease in cell viability, reduced ATP content, morphology changes of islets, and/or alterations of apoptotic biomarkers in a concentration- and/or time-dependent manner.
In contrast, silencing of Nrf2 sensitized MIN6 cells or islets to the damage.
In addition, pretreatment of MIN6 β-cells with NRF2 activators, including CDDO-Im, dimethyl fumarate (DMF), and tert-butylhydroquinone (tBHQ), protected the cells from high levels of H2O2-induced cell damage.
Given that reactive oxygen species (ROS) are involved in regulating glucose-stimulated insulin secretion (GSIS) and persistent activation of NRF2 blunts glucose-triggered ROS signaling and GSIS, the present study highlights the distinct roles that NRF2 may play in pancreatic β-cell dysfunction that occurs in different stages of diabetes.
American Psychological Association (APA)
Fu, Jingqi& Zheng, Hongzhi& Wang, Huihui& Yang, Bei& Zhao, Rui& Lu, Chunwei…[et al.]. 2015. Protective Role of Nuclear Factor E2-Related Factor 2 against Acute Oxidative Stress-Induced Pancreatic β-Cell Damage. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1075723
Modern Language Association (MLA)
Fu, Jingqi…[et al.]. Protective Role of Nuclear Factor E2-Related Factor 2 against Acute Oxidative Stress-Induced Pancreatic β-Cell Damage. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1075723
American Medical Association (AMA)
Fu, Jingqi& Zheng, Hongzhi& Wang, Huihui& Yang, Bei& Zhao, Rui& Lu, Chunwei…[et al.]. Protective Role of Nuclear Factor E2-Related Factor 2 against Acute Oxidative Stress-Induced Pancreatic β-Cell Damage. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1075723
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075723